Ankrd1 Mouse Gene Knockout Kit (CRISPR)

CAT#: KN301262

Ankrd1 - mouse gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: Ankrd1
• Locus ID: 107765
• Components:

  KN301262G1, Ankrd1 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: CCTAAGAGAAGGTGGTTGAG

  KN301262G2, Ankrd1 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: TCTTTCTCAGGAATAATGTG

  KN301262D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  CAGACATCAC TCCAGAGAAT TCCAAACAGA TAGAGACAAG TGGCACCCAG ACCCATCTCC TTCCCCTCGG GCTGATTATC CCCAGAAATA GGATGTCCCA AAGCAACACT TCCCAGCCAA CTGGAGTGCT GATAAGTCCA GTTATCAGAA AGATATGGCT GTAAGTGTGA TGCACAGTGC TTGCATTTTC TTGATACGTT AGTCATATGA GAGCTGACAA AGAAGGAAAA AGAGCAGCGA TGTGGTGCAA TATTAACAGG CAGCTGTCCC CTGGCTTCCC GATACGTGGG ATGACTCGCA TTGCTGAGCG GTGTGGTCAC TGCCAAAGGA ATGACCCTCT CACATTTCTT CCTGATTCGC ATACGCCGCG GCCAGCTTGT CATCTCCCTC TTGGGCTTCC CAGACACTAA GTCTGGAATG AAAATTCACC TGCCTCTGAA TTGGCCACTG GTGGGGGCAG GGGTGTGACT TGGCTTCCCA GGCTGGAAGA TTATCTCACC CAGCCCTAGC TATATAACGG GCTGGTGTGG AGGGGCTCCA CAGGGCCAGT TCCAGGGGTT CATCCACAAG AGAGAAAAAC ATAGACTCAC GGCTGCCAAC TGATGCTCAT CTTAATTGTG AGAGGAAGGC AGGCACCTGG GATACCTGGA GCTATGCTCC AGACAGTGCA CCGCCTGAAG GTGGCTGTCG CTGGATGGAG AGGAAGGACC TCCTGTGGCA TTGTTTGTGG ACAGTTACCC CAAGATGGGT AGCTTTGCTT CTGACTTCAA CATCTTCTTC ACATACAGGT AACAGGCAAA AAGAACAGCA ATGGGGCCGC AGGGGAATTC CTTCCTGGGG AGTTCAGAAA TGGAGAATAT GAAGCTGCTG TTGCTTTGGA GAAGCAAGAG GACCTGAAGA CACTTCCGGC CAACAGCGTG AAGCAGGGGG AGGAACAACG GAAAAGCGAG AAACTGCGAG AGGCAGAGGT GAGGACCTCC CACAAAATCC TAGTGGGAGT GGGGTACGGA GGTCCCTGAG GAAGAGGGGA AGAGAGGAGG AAAACAAATA AAAACTGCTC TAAAGATGTC TTTTCACCAG ATATGAAGCG CATGATGCAA AGAAGCAGTG CACATGGAAA TGACTGGGGG AATTACTTTT GGATTCCTCC TGTGTGGAGG GTTGAATCAA TGATTGAATG TGCTATGACC ACGTTTTGAT

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_013468
• UniProt ID: Q9CR42
• Synonyms: Alrp; CARP; Crap; MARP1
• Summary: May play an important role in endothelial cell activation. May act as a nuclear transcription factor that negatively regulates the expression of cardiac genes.[UniProtKB/Swiss-Prot Function]