TBLR1 (TBL1XR1) Human Gene Knockout Kit (CRISPR)

CAT#: KN222452

TBL1XR1 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: TBLR1
• Locus ID: 79718
• Components:

  KN222452G1, TBLR1 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: CAGTGATGAGGTCAACTTCT

  KN222452G2, TBLR1 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: TATAGATACTTGCAAGAGTC

  KN222452D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  ACTGTATTCC AGCCTATCTC ATACATACCT AGAGCTTTTT GAGACTTGAA CATTACAGAT GAGATGGTGA GAAAATAGAG ACTTCATATC CTGCCAAGTT TCATAGATAA AACTGGAGTG GATAACTCAT GGTTCTACCC CACCCAATGG TCTTTTCCCA AGTAACTGAT ATTCTTTTTT AACTAAATCA GCCTTCTTGC AAATAACTAA TATTTTTTAT TTTTAAAATT TACTTCTTTT TACAGTTAAG GAGAAAAAGT GAGCAACATT TTATGCTATT TGATAAAATC ATTAAAATAT TATGCTAAAG CCCTCTAAAA AATTTTGTTT TATTTTTGCC AGTATGTTAC TTTGAAGAAA AATAACACCA GCCATGTTTT GGTTGTATAA TCATTTTCCT AACTATTGGA AGCAACTTTG TCAGGTTTTA TTATTAGGCA ATGTAGAACC TTCGTTTTAC ATCATTTAAT AAAATCAAAT GGTTTTTACA ACATTGTTTA CATCTTGTTT AAAAATATTT TACTGAGAAA ATAATTAACT TTGTTTTATT TTTAGGGTTA TATCCTGTGT TGTGACCTCA TGGTTTAAGT GGGAATAAAG TAAATAGGCC TCAAATTATT TTAATATTCT CAAAATAATC TTTTATGCAT TTGAAATTTG TATCAGTCCA AACCGTTGAA CTTTGTATGT TTTCTTAATG TCTGGACTGC CATTTTAAAG CTATGTTTTA CTGCATGGTT TAACTCTCTG ACTCTTTCAA ATATGTTGCA AACAAGGCAA TTATTTGTAA CAGTTTGGCT TTCGTTGTTC AAAGACCATT TTTATAATCT GTTATGAGGT GAGATTATTG CATATGTAGT AGGAACATAA AAACTTTTAG CCACCATCAG TTTGATAATG TATATCCAGA ATTCTCAATT AAATGTTGTT GGTCCTAATT GTAGTTAGTA TTTTGGAAAT TAAATTAACA GTATTTTCCT AGAATGCTTT CAAAATTATT ATTTGAATTA AGTGTATCCT TGATCTGAAT TGTTATTATT GTAATGGGAG TAATTATAAA TAGAAATCAT TGAAAGCCAT TTGCAACTAA TATTAAATCA ATCAGTGATC TTCCAAGTTT TATTGATAGA TAAAACAGAA GGAATACTGC AAGGGGAAAT GTGGATGGAT TAAGCAAAAA TTTAAAAGTA GAAGAGGGAA

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001321193, NM_001321194, NM_001321195, NM_024665
• UniProt ID: Q9BZK7
• Synonyms: C21; DC42; IRA1; MRD41; TBLR1
• Summary: This gene is a member of the WD40 repeat-containing gene family and shares sequence similarity with transducin (beta)-like 1X-linked (TBL1X). The protein encoded by this gene is thought to be a component of both nuclear receptor corepressor (N-CoR) and histone deacetylase 3 (HDAC 3) complexes, and is required for transcriptional activation by a variety of transcription factors. Mutations in these gene have been associated with some autism spectrum disorders, and one finding suggests that haploinsufficiency of this gene may be a cause of intellectual disability with dysmorphism. Mutations in this gene as well as recurrent translocations involving this gene have also been observed in some tumors. [provided by RefSeq, Mar 2016]