OVCA1 (DPH1) Human Gene Knockout Kit (CRISPR)

SKU KN221955BN

DPH1 - human gene knockout kit via CRISPR, HDR mediated

Product Data

• Format: 2 gRNA vectors, 1 mBFP-Neo donor, 1 scramble control
• Vector: mBFP-Neo
• Target Symbol: OVCA1
• Locus ID: 1801
• Components:

  KN221955G1, OVCA1 gRNA vector 1 in pCas-Guide CRISPR vector

  KN221955G2, OVCA1 gRNA vector 2 in pCas-Guide CRISPR vector

  KN221955BND, donor DNA containing left and right homologous arms and mBFP-Neo functional cassette.

  GE100003, scramble sequence in pCas-Guide vector

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001383, NM_001346574, NM_001346575, NM_001346576, NR_144474, NR_144475, NR_144476
• UniProt ID: Q9BZG8
• Synonyms: DPH2L; DPH2L1; OVCA1
• Summary: The protein encoded by this gene is an enzyme involved in the biosynthesis of diphthamide, a modified histidine found only in elongation factor-2 (EEF2). Diphthamide residues in EEF2 are targeted for ADP-ribosylation by diphtheria toxin and Pseudomonas exotoxin A. Defects in this gene have been associated with both ovarian cancer and autosomal recessive intellectual disability with short stature, craniofacial, and ectodermal anomalies. [provided by RefSeq, Oct 2016]
• Shipping: Ambient