ST8SIA2 Human Gene Knockout Kit (CRISPR)

CAT#: KN219160

ST8SIA2 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: ST8SIA2
• Locus ID: 8128
• Components:

  KN219160G1, ST8SIA2 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: CTGAGATGTCTGCGAAGATG

  KN219160G2, ST8SIA2 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: CAGAGATCGAAGAAGAAATC

  KN219160D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  CTGGGGGCTG TCCCCAGGTG TTGGTCTTTG TGTCGAGAAA ACTTTGCGAG GAGGCGAGGG AGCTGGACAA AGAGCGGGCG AGGGAGGGCG TTTGCCAGCC TGAGGGGTGC GCCGGGCGGT GCGCGCACTT CCCTGGGCTC AGCTCCGTAC CCGGTCGCTC GCGGTGGCCA GAGCCCAGTC TCTCTAGGCC AGGAGTGGCG TCCGGCTGCA TCCCCGCGCC CTCGGGAGCT GAATAAAGCC AGGCGGGAGA AGCAAAGGCT AGCGCCTGGA CGCTGGAGTT GGTGGGAGGA GGCGAGCAGA GGCGCGGAGC AAAGCTGTCA AACTGCGCGC GGAGCCGGGC GCGGGGCGGG GGAAGGGAGA GGAGGGAGGG AGGGGCAGGC GGGAGCCGGG AGGGAGCGAG CGGGCTCGCC GCGCCTGAGC AACCCCTGCC TGTCGCTGCC GCTGCCGCTG CCGCTGCCGC CGCCGGCCCG GACTCGTCCG GAGCGCAGGG TGTCTGCCCA GCTGCGCGCG GCGCGCGGAG GCTCCGGCGT CCGCCGCTGC GCCCTCCGGC CCCTGCTCCT CGCGCCGGCC CGCGTGGGTC CCGGCGGGCG CGAACCCACC CCCGTGCCAC GAGCCCTGGC GGGATCTCTC CCTCCTTCTG TACTCTCCAC CCTCCGACCC CCTTTGTGTG CGCCGCGCCC CGCTGTAGCT CCGCTGGAGA GATGGGGACG GTTTGGCAGA AGGTGGCGGC GACAGGGGCC CGGGACGGTT TGGGGAGAGG AGGGTGGGGG CGCGGCTGCT GTGGGGTGTC CCCGGGCGGG GTTCACGGGA AGCCCCCAGC TGGCCCGCGT CGGGAAGCGG ACTCGCCCAC CCTGCCCCCC TGCCTGGCGT GTCCAAGCGC AGTGTCGCCG GGGTCGAGGG AGGGCGCCGG GGAGTGTCTC GGGCGGGCGG AGGGGCCGAG AGTGGAGGGA GGCAGGAGTT TCTTTGTCTC CAGCACGGCC GCTTTCTCGC CTTGGCAAAG TGGGCAGGGG GCGCGCAGGG ATCTCTGAGC TGGGGGAGGG GTTCCTGGGG CCGGAGCGGA GGGCGGGGTT CTGCATGGGC TACTGGGAAG CGGAATGTCC CCTGCACACA CAGACAGGCA CACGCGCCTC CCGCCGGCCC GGGAGCCCGG CGTAGGAGCT GGCACCACTA GAGAAGGGCT CGCATAGCTG GACTGAGACC

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_006011, NM_001330416
• UniProt ID: Q92186
• Synonyms: HsT19690; SIAT8B; ST8SIA-II; STX
• Summary: The protein encoded by this gene is a type II membrane protein that is thought to catalyze the transfer of sialic acid from CMP-sialic acid to N-linked oligosaccharides and glycoproteins. The encoded protein may be found in the Golgi apparatus and may be involved in the production of polysialic acid, a modulator of the adhesive properties of neural cell adhesion molecule (NCAM1). This protein is a member of glycosyltransferase family 29. [provided by RefSeq, Jul 2008]