Growth hormone receptor (GHR) Human Gene Knockout Kit (CRISPR)

CAT#: KN218710

GHR - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: Growth hormone receptor
• Locus ID: 2690
• Components:

  KN218710G1, Growth hormone receptor gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: ACTTGATCCTGCCAGTGCCA

  KN218710G2, Growth hormone receptor gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: TTCGCTACATCCAAGTTTTT

  KN218710D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  TTAGTTTTAC TAGGGAGTTT TTGTTTCCTT TTGCTTCCCT CCTTCTTTCT GCTTCCCTTC TAGTTAAACC TCTTTAGGAT GGCTCATTAG CAACTCGTTT TGAGTGGTTT GAGCTCTTTT GTTCACTGGG AAACAGATTT ATGAAATGTT ACTATTACCA TGATTGTTAT GTTTTTCCTT TTATGGCCTG TCCAGCTCAA GGCCCCATGC TTTCTGATAC TGCTGATAAG GTTTTCTATT TCCAGATCAA ATTAAAGCAA ACCTTACTGG CCCTGTTACT GAAGCTGTGC ATGGGGGTCG TTTGCTGTGA GGTGTTTCTA TGGCTTTGAG CCAGGGTATG AACATCTCTA GTGTTCATGT TATTTCCTGA GACTAGCACT CACGGGAAGT AGAATTTATT ACAACCTGCT GTTTGAGTTC ATGAAAAGTA GGACAATATG AGACTCTGGG GCAGTGAAAG ACTTACCAGG ATTCCTTCTG GAACTGACTC GTCAGCTCAT TCATGTCTTA CCCAGTCTTT AAACAGTATT TCATGATAAT GGTCTGCTTT TAATTGCTGG GCTTTACCTT ACCCTTTTTG TGATTGCAGG TCCTACAGGT GGATGATTTT ATTAAAAGAT GCAAGTTTTA CATAGCAGCA AAAAGGAAAC TTGACTTAGC TTTAAAATCA GAAACTAGTA GGACTTTTCT GTGGATGTTT AGGAGGAATG CAGTAGTCAG TCAACTTTGG CATCTGATGT TTTCATATCA AAAATTAATA CTACAAATCA TGCTGAGGAC ATTTATGTTG AAGACAAGCC AGGTCTCTTG TTCTCAGGCT TTGGGAGAGA TATTGAAATT ACACTGAAGG GTTATTAGCT TACTGTTCAC TCAGCTCATC TGTGACTAAG GATATCTAAT CTATTTCCAG AGCTCAGAAT TCAGGCTTGT TAATCATATT CTCAGAGAAA AGAAATTGCA GGTTTCAAAT GAATTTCACT TCTCACTCTC TGGGTGATTA CCATTTGGCT AAGTTGAGGA AAGAGACACC TGAAATAGTT GAATGATATA CCCAGAAATT ATGAGCTTCC TTCCCCATCT CTATAATTCT CCCTCCCTTT TTTATATCCC TCTCCTTGTA GAGAAGTCTC AATATGTTTA AACTATGTTT CATAGCAGAC CTAACTAAAA CAAGGAAGAG TAATAGAAGG GAAAGGGAAG

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_000163, NM_001242399, NM_001242400, NM_001242401, NM_001242402, NM_001242403, NM_001242404, NM_001242405, NM_001242406, NM_001242460, NM_001242461, NM_001242462
• UniProt ID: P10912
• Synonyms: GHBP; GHIP
• Summary: This gene encodes a member of the type I cytokine receptor family, which is a transmembrane receptor for growth hormone. Binding of growth hormone to the receptor leads to receptor dimerization and the activation of an intra- and intercellular signal transduction pathway leading to growth. Mutations in this gene have been associated with Laron syndrome, also known as the growth hormone insensitivity syndrome (GHIS), a disorder characterized by short stature. In humans and rabbits, but not rodents, growth hormone binding protein (GHBP) is generated by proteolytic cleavage of the extracellular ligand-binding domain from the mature growth hormone receptor protein. Multiple alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jun 2011]