DDI2 Human Gene Knockout Kit (CRISPR)
CAT#: KN218468RB
DDI2 - human gene knockout kit via CRISPR, HDR mediated
Functional Cassette: GFP-puro Luciferase-Puro mBFP-Neo
HDR-mediated knockout kit validation
USD 1,657.00
4 Weeks*
Specifications
Product Data | |
Format | 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control |
Donor DNA | RFP-BSD |
Symbol | DDI2 |
Locus ID | 84301 |
Components |
KN218468G1, DDI2 gRNA vector 1 in pCas-Guide CRISPR vector KN218468G2, DDI2 gRNA vector 2 in pCas-Guide CRISPR vector KN218468RBD, donor DNA containing left and right homologous arms and RFP-BSD functional cassette. GE100003, scramble sequence in pCas-Guide vector |
Disclaimer | These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process. |
Reference Data | |
RefSeq | NM_032341 |
UniProt ID | Q5TDH0 |
Synonyms | RP4-680D5.5 |
Summary | Aspartic protease that mediates the cleavage of NFE2L1/NRF1 at 'Leu-104', thereby promoting release of NFE2L1/NRF1 from the endoplasmic reticulum membrane (PubMed:27676298, PubMed:27528193). Ubiquitination of NFE2L1/NRF1 is a prerequisite for cleavage, suggesting that DDI2 specifically recognizes and binds ubiquitinated NFE2L1/NRF1 (PubMed:27528193). Seems to act as a proteasomal shuttle which links the proteasome and replication fork proteins like RTF2 (Probable). Required, with DDI1, for cellular survival following replication stress. Together or redudantly with DDI1, removes RTF2 from stalled forks to allow cell cycle progression after replication stress and maintains genome integrity (PubMed:29290612).[UniProtKB/Swiss-Prot Function] |
Documents
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
KN218468 | DDI2 - human gene knockout kit via CRISPR, HDR mediated |
USD 1,657.00 |
|
KN218468BN | DDI2 - human gene knockout kit via CRISPR, HDR mediated |
USD 1,657.00 |
|
KN218468LP | DDI2 - human gene knockout kit via CRISPR, HDR mediated |
USD 1,657.00 |
|
KN418468 | DDI2 - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated. |
USD 1,657.00 |
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GA113496 | DDI2 CRISPRa kit - CRISPR gene activation of human DNA damage inducible 1 homolog 2 |
USD 1,657.00 |
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