C19orf80 (ANGPTL8) Human Gene Knockout Kit (CRISPR)

CAT#: KN218000

ANGPTL8 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: C19orf80
• Locus ID: 55908
• Components:

  KN218000G1, C19orf80 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GCCAGTTCTGGGCCGCCCAT

  KN218000G2, C19orf80 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: CAGGCCGGGTCACCATTGCC

  KN218000D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  AACATCACCG CCATTTTACA AGCAGGGAAA CTGAGGTACT TGCACAATCA CCCACGGAGT AAAATGGCAG TCAGTGGTGA TCTGGGCATA TGCAGGTCTT GGAGATCAGG CTGTGTGACC TTGGGAGAGT CTCTGCCACC TGCACCTGCC CCCTTGGATA CTAACCAGGC CAATAAAAGC CCCCACGTGG CAAGTATGTG GGAGGGGAAC AAGAGCAGAT ATTGTAATGG CAGGTCAAAC AGCTCAGGGC AAACCATCAA TGCTTATAAA ATCTTTACTG TTATTACCAT CATCATTGCG ATGCCTATTC ATAGATGGAG AAACAGGCTC AGCATGGTGA GTCTCAGGAA CTCCCAGCAG GGTCTGGGGC CCGTCCTCTC CCAATGCCCC CCACCCACAC CTGGCCCTGA GCCCCAGGAA GCGTGATCCC GGCGTGGCCT AGCCGGACAT TCGCCTGATG CAACTATCGC ACCACTGCCA GGCCCTTGTG CAATGCGGGG CACCAGGTCG GCCAGTTAAC GATTGACTGG GGATCAGTGG GGGTATGAGG CATAAAAGGC TTCAGTGACA GTGCTGTGGC TATACCTTAG ACCCTCAGTC CAGCTGGGCC AGGCCCTCAA CGGTGTGTAC AGGACCACGG AGGGACGGCT GACAAAGGCC AGGAACAGCC TGGGTCTCTA TGGCCGCACA ATAGAACTCC TGGGGCAGGA GGTCAGCCGG GGCCGGGATG CAGCCCAGGA ACTTCGGGCA AGCCTGTTGG AGACTCAGGT GGGCACCGTA GCTGCGACAC TGTGGGGTGG CCAGGAGTCC AAAGAGGAGT TCGTGTCTAG GGTAACCAAC CATCCTGGTT TGCCCAGGAC TGAAGGGATT CCTGGGATAC AAGATTTTCA GCGATAAACT CAGGCAAGTC CTTAGGTACA CAAAGATGAG TTGGACATCC TACTAGTGAC CCACTGTTTA TTAAGCAGAT GGAGGAGGAT ATTCTGCAGC TGCAGGCAGA GGCCACAGCT GAGGTGCTGG GGGAGGTGGC CCAGGCACAG AAGGTGCTAC GGGACAGCGT GCAGCGGCTA GAAGTCCAGC TGAGGAGCGC CTGGCTGGGC CCTGCCTACC GAGAATTTGA GGTCTTAAAG GTAAGGAGCT CCCCCAACCC TAGTGGGCTG AGACCCTGAT TTCCGGCCAG AACTCGCTTC TGCACCTTGA

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_018687
• UniProt ID: Q6UXH0
• Synonyms: C19orf80; PRO1185; PVPA599; RIFL; TD26
• Summary: Hormone that acts as a blood lipid regulator by regulating serum triglyceride levels (PubMed:22569073, PubMed:22809513, PubMed:23150577). May be involved in the metabolic transition between fasting and refeeding: required to direct fatty acids to adipose tissue for storage in the fed state (By similarity).[UniProtKB/Swiss-Prot Function]