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DNMT1 Human Gene Knockout Kit (CRISPR)

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SKU
KN217851
DNMT1 - human gene knockout kit via CRISPR, HDR mediated
  $1,657.00
4 Weeks*

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Other products for "DNMT1 or NM_001130823"

Specifications
Specifications
Product Data
Format 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Vector GFP-puro
Target Symbol DNMT1
Locus ID 1786
Components

KN217851G1, DNMT1 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: CGGGCTGGGGCGGTACGCGC

KN217851G2, DNMT1 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: TACCGCCTGCGGACATCGTC

KN217851D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

Homologous arm and GFP-puro sequences:

pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

AAAAAGGCAA CAAAAAACCC CCCACACACT GGGTATAGAA GTGGCATGGG CCTCTATACA CTGTGAGATT CTTGGTACTA GCTACAAATT CTGTGTATAC TCAAGATTTT CTAGAGTAGG TGCAATTACC CCGTTTTACA GATGAGGACA CAGAGGCTGA GCCGTAGTGA CCCACCTAAG GTCGTATAGC CAGCAAATAG ATGGAGGTTG GATTGGAACT GAGGACTTTA CTCAAGGGCT CTCACAAACC CTTGGGGGCT TCTCGCTGCT TTATCCCCAT CACACCTGAA AGAATGAATG AATGAATGCC TCGGGCACCG TGCCCACCTC CCAGCAAACC GTGGAGCTTG GACGAGCCCA CTGCTCCGCG TGGGGGGGGT GTGTGCCCGC CTTGCGCATG CGTGTTCCCT GGGCATGGCC GGCTCCGTTC CATCCTTCTG CACAGGGTAT CGCCTCTCTC CGTTTGGTAC ATCCCCTCCT CCCCCACGCC CGGACTGGGG TGGTAGACGC CGCCTCCGCT CATCGCCCCT CCCCATCGGT TTCCGCGCGA AAAGCCGGGG CGCCTGCGCT GCCGCCGCCG CGTCTGCTGA AGCCTCCGAG AACACGGACT CAGGGGGACA GGCAGGGCGC TGGGTGGGGG GTCGCTTCCC CTCGGGGTGG CCGGTGGCCG CTGCTGACAG ACGGGCGCGC ATGCGTGGGG TGGTGCGGCG CGCAGCGGCA GTTGGCGCGG GCAGGGTGGC ACTTCCGGTC GCGCGTGCCC GGGCTGTTTG GCGCCAAAAT GGACCGTGGA TTCCCCCGTA GCTCCCTGGT GGCTAGAAAC TAGGCGGGGT GGGGGTTCTC TTTTGATCCC CAAATACAGC AAGCTTTGGG TTCGTTTCCG GGGTCCCCTT CTTCAAGCAG CGGTGGGCCG GGCTCTTGAC TCCAGCTTGG TCGCACAGGA AGTGGGCAGC CCGCGGCCAA CGGACACCCC TCGGGCACCG GCACTTCGGC CTCCACTTCC GGTGTCCGGC CCGGTCCCCG GGGGCGCTTC TGTGGTGGGG GGTCCCTCAG TGCCTTTCCC CCAAAGCTGT GCATCTCGAT GGCGGCCTCC AGAGACGGAT GTCACCCGTC GGGGCCTGGG CTGGGCTGGC TCATGTTCGA GAGCCCAGCC TTCCGTGGGG TACTCATTCT GTCAGTTGGG ACTGTGTGGT TCTTGCTCCT

GE100003, scramble sequence in pCas-Guide vector
OTI Disclaimer These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq NM_001130823, NM_001318730, NM_001318731, NM_001379
UniProt ID P26358
Synonyms ADCADN; AIM; CXXC9; DNMT; HSN1E; m.HsaI; MCMT
Summary This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants. provided by RefSeq, Jan 2016
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Documents
Resources
Other Versions
SKU Description Size Price
GA101242 DNMT1 CRISPRa kit - CRISPR gene activation of human DNA methyltransferase 1 1 kit
$1,657.00
KN217851BN DNMT1 - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN217851LP DNMT1 - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN217851RB DNMT1 - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN417851 DNMT1 - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated. 1 kit
$1,657.00

file-alt Citations

Endoplasmic reticulum protein 29 (ERp29) confers radioresistance through the DNA repair gene, O(6)-methylguanine DNA-methyltransferase, in breast cancer cells
Chen, Zhang, Zhang
Sci Rep (2015) 5, 14723 DOI: 10.1038/srep14723
Cited by 22
AI Summary: OriGene’s Trilencer-27 Human siRNA kits targeting ERp29, MGMT, and DNMT1 were used in this study to selectively knock down these genes and investigate their roles in radioresistance and DNA repair in breast cancer cells.
Snippet: ERp29 Trilencer-27 Human siRNA, MGMT Trilencer-27 Human siRNA and DNMT1 Trilencer-27 Human siRNA kits include three Dicer-Substrate 27-mer duplexes for each gene and were purchased from Origene (Rockville, MD). ... Cite SaveSaving
FigureFigureFigure
Showing 1-1 of 1 papers.
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