UGT2B28 Human Gene Knockout Kit (CRISPR)

CAT#: KN216435

UGT2B28 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: UGT2B28
• Locus ID: 54490
• Components:

  KN216435G1, UGT2B28 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: CATCCAATGGCTGTATTCAC

  KN216435G2, UGT2B28 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GGAAAGGTGCTGGTGTGGAC

  KN216435D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  AGAAATTAGA TTACTGGTTG CCCAGTGCAG GGCGGGTGCA GAAATTTGGT GGGGAGATGA GAGCTAATAC ACAGGAGTTT TTTGTTTTTT TAGATAAAAC TGATTGTTAT GTTGGATGCA CAAGTCTGTG AATTTACAAG GAAATACTTA ATTTTAAATA CTAATTGATG AATCATATGG TATTTGAATT ATACTTCAAC AAAGTTTTAC AAGATATTCC TTGAACCACG ATATTCAAAA ACGCTATTTG CATTGTACGA TCACTTCATC TCTTACTCTG TGTCAAGGGC CCTGCAGGAC TCTCTCACTT GCCACTATTC TTGACACTAT TATAAAATAG GTTACATAAA ACAGGGTACT TGCAAGGTCA TTAATCTTAG GTGGATGACA GCGAATATAA ACATAAAAGA AACATCAAAC ATCCTTGAGT AAATATGAAG TAATCAAACT TTGTCTCCTT GTCATCCATG TACTCAGGAT GCTCCTATAA TATATTTACT TTGGATTGAA GGAGTTATGT TTTAACTTGA TTGATTTATC TCTGTATATA AGTATGAGAA AGAAACAGTG ACTTGAAAAG AATGATTGCA TTGCACCAGG AGCTTGTTCA GAGAGGTCAT GAGGTGACTG TACTGGCATC TTCAGCTTCC ATTCTTTTTG ATCCCAATGA CGCATTCACT CTTAAACTCG AAGTTTATCC TACATCTTTA ACTAAAACTG AATTTGAGAA TATCATCATG CAACAGGTTA AGAGATGGTC AGACATTCAA AAAGATAGCT TTTGGTTATA TTTTTCACAA GAACAAGAAA TCCTGTGGGA ATTTCATGAC ATATTTAGAA ACTTCTGTAA AGATGTAGTT TCAAATAAGA AAGTTATGAA AAAACTACAA GAGTCAAGAT TTGACATCAT TTTTGCAGAT GCTTTTTTTC CTTGTGGTGA GCTGCTGGCT GCGCTACTTA ACATACCGTT TGTGTACAGT CTCTGCTTCA CTCCTGGCTA CACAATTGAA AGGCACAGTG GAGGACTGAT TTTCCCTCCT TCCTACATAC CTGTTGTTAT GTCAAAATTA AGTGATCAAA TGACTTTCAT GGAGAGGGTA AAAAACATGA TCTATGTGCT TTATTTTGAC TTTTGGTTCC AAATGTGTGA TATGAAGAAG TGGGATCAGT TTTACAGTGA AGTTTTAGGT AAGAATTTGT

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001207004, NM_053039
• UniProt ID: Q9BY64
• Summary: This gene encodes a member of the uridine diphosphoglucuronosyltransferase protein family. The encoded enzyme catalyzes the transfer of glucuronic acid from uridine diphosphoglucuronic acid to a diverse array of substrates including steroid hormones and lipid-soluble drugs. This process, known as glucuronidation, is an intermediate step in the metabolism of steroids. Two transcript variants encoding different isoforms have been found for this gene. While both isoforms are targeted to the endoplasmic reticulum, only the longer isoform appears to be active. [provided by RefSeq, May 2011]