MPIG6B Human Gene Knockout Kit (CRISPR)

CAT#: KN215589

MPIG6B - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: MPIG6B
• Locus ID: 80739
• Components:

  KN215589G1, MPIG6B gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: CCCTGGGGGTAAGCGATCCC

  KN215589G2, MPIG6B gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GAGAGTTGTGATAGACGCAG

  KN215589D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  GCCGGAGGCT GCAGTGAGTC GAGATTGCAC TCCAGCCTGG CCACAGAGCA AGACTCTGAC TCAAAAAAAA AAAAAAAAAA AAGAATAGAA ATAGTAATAA TAATGGCAAG CACTTACATA GTGATCCTAT GTATTCTAAG CAGTTTACAT GTATTACTTT ATTTCGTTAT CACAATCCCC TACAAAACAG GAGTTTTTTT TGTTGTTGTT TTTGAGACAG GGTCTGGCTG GCTCTGTCGC CCAGGCTGGA GTGCAATGGC CTGATCACAG TTCACTGCAA CCTCGACCTC CTGAGCTCAA GCGATCCTCC CTCCTCAGCC TCCTAAGTAG CTGGGATTAC AGGCGCACCT GAAAAGTTAA GCAGGCCAAA GCATTTGTGT AAATGGCCCG AGCACACATT TTAAGTGAGA ACCATTTGAA GACTCCGAGT TTGCCTGCGA GGATTCCCAA AGGGATCTGG GCAGCTGGTG GCCCCGCCCC CTCTCTTATC GGAGCCCCCC AGCCCCTCCG TTCTCCCCAC GCCTAACTTC CCTCCGGTCC CCCCCCAACC GGCCCCACGC CGCTGATTCG CTCGCAGCTT CTCCTCACCA CATCCTAACC AGGGGCTGAA GCAAGATAAG GGCCGCCTAG TAGGGTGGGT TGTGTGTGGG AAGATCCAGG ATGGCTGGAG TGCAGAACAG AGAAGAGAAA GAGGAGACGG GATGGAGGGT CGTCTTGCCC TGTGGACGTG CCCTAACCAC AGCCTCCGGC CTCTCCTAGC TTCTCTGGAC GGCCGCCCTG GGGACCGGGT GAATCTCTCC TGCGGAGGAG TCTCTCATCC CATCCGCTGG GTCTGGGCAC CCAGCTTCCC GGCCTGCAAG GGCCTGTCCA AAGGACGCCG ACCGATCCTG TGGGCCTCTT CGAGCGGGAC CCCCACCGTG CCTCCCCTCC AGCCTTTCGT CGGCCGCCTA CGCTCCCTGG ACTCTGGTAT CCGGCGGCTG GAGCTCCTCT TGAGCGCGGG GGACTCGGGC ACTTTTTTCT GCAAGGGCCG CCACGAGGAC GAGAGCCGTA CAGTGCTTCA CGTGCTGGGG GACAGGACCT ATTGCAAGGC CCCCGGGCCT ACCCATGGTA GGTGCAGGCC TGTGCGCACA AGGGTACTTA ACTCCGACAC ATACCCGGAG GAGGGAAGAG GGCCTTGGCT GGGGGTTCTT GAGCGGGACT

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_025260, NM_138272, NM_138273, NM_138274, NM_138275, NM_138276, NM_138277
• UniProt ID: O95866
• Synonyms: G6b; NG31
• Summary: This gene is a member of the immunoglobulin (Ig) superfamily and is located in the major histocompatibility complex (MHC) class III region. The protein encoded by this gene is a glycosylated, plasma membrane-bound cell surface receptor, but soluble isoforms encoded by some transcript variants have been found in the endoplasmic reticulum and Golgi before being secreted. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]