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PIK3CB Human Gene Knockout Kit (CRISPR)

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SKU
KN215433
PIK3CB - human gene knockout kit via CRISPR, HDR mediated
  $1,657.00
4 Weeks*

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Specifications
Specifications
Product Data
Format 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Vector GFP-puro
Target Symbol PIK3CB
Locus ID 5291
Components

KN215433G1, PIK3CB gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: CATCCTTGACATCTGGGCGG

KN215433G2, PIK3CB gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: ATCTGATGGCTCCATACCTG

KN215433D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

Homologous arm and GFP-puro sequences:

pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

TCTCAAAAAA AAAAAAAATT GTCCTTGTAG AGAAAGGGCC CTCTTGATTG CAAATTGTTC TTGTTGAGAG AGGGCCCTCT TGATTGTCAT TTTCATTGGA ATTTGCAGAG AAAAACTCAG CCTAAGAAGC TAATATTTTC TAAATAAAGT TTGGAGAAAA ATCACCTGTC AAGTGCTGGT TAACTAAATT TCTTAGGACC CTGTAATCCT TAAGCATGCA GTTTATTCAG ATGTCAAATA TCTATGTTGA ATTTGAATCA ATGTGGTGTG TGTGTGTGTA CATATGTATG TGTATATATT TTTATTTATA TGTATATAAG AATAATTTTT TTCCTTTAGT TGGTGGTATG GATTGGATAA TGTACATATA TATAATTTGT GAAGTGATAA AAGTGTATTT AAATAATTTA TTTCTGCTGA CTTACTCTTG GTTTTTAAAA TATCACATTA TTTAATTGGC CTCCTGTTAC ATTATTGGCT ATATTGAAAT CTAAGGACTA ATTGTAGATT AGTGGTCTTT ATCATATCTT GGTATAAAAT TTGGCTGATG GTTGTTTTGC TATGTTTTAA TTACTGATTT CTAGGGCCCC GTGGTTATGA GGGATTTATA TCCAGTTGGA GGTACCTCGG GAAGCTACCA TTTCTTATAT TAAGCAGGTA TGTTAATAGG TATAATTTCT ATGTGTTTAG CATACTGTGT CACTTGGGGG AAATATCATT CTATAATAGA AAATGTTAAT GTAAATTATA TGTGATAATG ATAGTAATGA AGTGATGGTA ATTAAGAAAA ATTAGTTTAT GTTAGCTAGT TTGAAATGTT TGTTTGGGTT ATTAAAACTG TCAAGAAGAT TTTCTTTTGA GTTCGTCTTT GAAATTACTG TCTTTGAGCT TGTCACACTT TTAAAAATGT GATTTTTGGT TCATTTTCTC TTCTCTCCTT TTTCTTCTCT TCCCCCTTTC CTTGACTCTT GGGGGAAGAA AGACTTTTCT GCTGTTCACC AGATATTTTT CCCTCAGCAG GCTTGCCTTT TCTGTTGCAT TAGTTTTTAA AGTAAATTGA TGAGCACCGT TGATTTGTAA AAACCAGCAT ATACATGAAC TTGTTTTTCT AAACTTTCTT TCTAATGTTT TTCAAAAAGC TGAACCCAAT GTGAATATCA GGAGTTACGG TATTTTTAAG AAAACATATT TATGATATAC

GE100003, scramble sequence in pCas-Guide vector
OTI Disclaimer These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq NM_001256045, NM_006219
UniProt ID P42338
Synonyms P110BETA; PI3K; PI3KBETA; PIK3C1
Summary This gene encodes an isoform of the catalytic subunit of phosphoinositide 3-kinase (PI3K). These kinases are important in signaling pathways involving receptors on the outer membrane of eukaryotic cells and are named for their catalytic subunit. The encoded protein is the catalytic subunit for PI3Kbeta (PI3KB). PI3KB has been shown to be part of the activation pathway in neutrophils which have bound immune complexes at sites of injury or infection. Alternative splicing results in multiple transcript variants. provided by RefSeq, Dec 2011
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Documents
Resources
Other Versions
SKU Description Size Price
GA103556 PIK3CB CRISPRa kit - CRISPR gene activation of human phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta 1 kit
$1,657.00
KN215433BN PIK3CB - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN215433LP PIK3CB - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN215433RB PIK3CB - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN415433 PIK3CB - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated. 1 kit
$1,657.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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