PIK3CB Human Gene Knockout Kit (CRISPR)

CAT#: KN215433

PIK3CB - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: PIK3CB
• Locus ID: 5291
• Components:

  KN215433G1, PIK3CB gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: CATCCTTGACATCTGGGCGG

  KN215433G2, PIK3CB gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: ATCTGATGGCTCCATACCTG

  KN215433D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  TCTCAAAAAA AAAAAAAATT GTCCTTGTAG AGAAAGGGCC CTCTTGATTG CAAATTGTTC TTGTTGAGAG AGGGCCCTCT TGATTGTCAT TTTCATTGGA ATTTGCAGAG AAAAACTCAG CCTAAGAAGC TAATATTTTC TAAATAAAGT TTGGAGAAAA ATCACCTGTC AAGTGCTGGT TAACTAAATT TCTTAGGACC CTGTAATCCT TAAGCATGCA GTTTATTCAG ATGTCAAATA TCTATGTTGA ATTTGAATCA ATGTGGTGTG TGTGTGTGTA CATATGTATG TGTATATATT TTTATTTATA TGTATATAAG AATAATTTTT TTCCTTTAGT TGGTGGTATG GATTGGATAA TGTACATATA TATAATTTGT GAAGTGATAA AAGTGTATTT AAATAATTTA TTTCTGCTGA CTTACTCTTG GTTTTTAAAA TATCACATTA TTTAATTGGC CTCCTGTTAC ATTATTGGCT ATATTGAAAT CTAAGGACTA ATTGTAGATT AGTGGTCTTT ATCATATCTT GGTATAAAAT TTGGCTGATG GTTGTTTTGC TATGTTTTAA TTACTGATTT CTAGGGCCCC GTGGTTATGA GGGATTTATA TCCAGTTGGA GGTACCTCGG GAAGCTACCA TTTCTTATAT TAAGCAGGTA TGTTAATAGG TATAATTTCT ATGTGTTTAG CATACTGTGT CACTTGGGGG AAATATCATT CTATAATAGA AAATGTTAAT GTAAATTATA TGTGATAATG ATAGTAATGA AGTGATGGTA ATTAAGAAAA ATTAGTTTAT GTTAGCTAGT TTGAAATGTT TGTTTGGGTT ATTAAAACTG TCAAGAAGAT TTTCTTTTGA GTTCGTCTTT GAAATTACTG TCTTTGAGCT TGTCACACTT TTAAAAATGT GATTTTTGGT TCATTTTCTC TTCTCTCCTT TTTCTTCTCT TCCCCCTTTC CTTGACTCTT GGGGGAAGAA AGACTTTTCT GCTGTTCACC AGATATTTTT CCCTCAGCAG GCTTGCCTTT TCTGTTGCAT TAGTTTTTAA AGTAAATTGA TGAGCACCGT TGATTTGTAA AAACCAGCAT ATACATGAAC TTGTTTTTCT AAACTTTCTT TCTAATGTTT TTCAAAAAGC TGAACCCAAT GTGAATATCA GGAGTTACGG TATTTTTAAG AAAACATATT TATGATATAC

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001256045, NM_006219
• UniProt ID: P42338
• Synonyms: P110BETA; PI3K; PI3KBETA; PIK3C1
• Summary: This gene encodes an isoform of the catalytic subunit of phosphoinositide 3-kinase (PI3K). These kinases are important in signaling pathways involving receptors on the outer membrane of eukaryotic cells and are named for their catalytic subunit. The encoded protein is the catalytic subunit for PI3Kbeta (PI3KB). PI3KB has been shown to be part of the activation pathway in neutrophils which have bound immune complexes at sites of injury or infection. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011]