NSUN2 Human Gene Knockout Kit (CRISPR)

CAT#: KN214459RB

NSUN2 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control
• Donor DNA: RFP-BSD
• Symbol: NSUN2
• Locus ID: 54888
• Components:

  KN214459G1, NSUN2 gRNA vector 1 in pCas-Guide CRISPR vector

  KN214459G2, NSUN2 gRNA vector 2 in pCas-Guide CRISPR vector

  KN214459RBD, donor DNA containing left and right homologous arms and RFP-BSD functional cassette.

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001193455, NM_017755, NR_037947
• UniProt ID: Q08J23
• Synonyms: MISU; MRT5; SAKI; TRM4
• Summary: This gene encodes a methyltransferase that catalyzes the methylation of cytosine to 5-methylcytosine (m5C) at position 34 of intron-containing tRNA(Leu)(CAA) precursors. This modification is necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Mar 2011]