ADCY7 Human Gene Knockout Kit (CRISPR)

CAT#: KN214351

ADCY7 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: ADCY7
• Locus ID: 113
• Components:

  KN214351G1, ADCY7 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GGGCGCTACTTCCTCAACGA

  KN214351G2, ADCY7 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: CGTAGAGCGCATCTTGGTCA

  KN214351D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  TGAGGTGGGA GAATCACTTG AGCCCGGGAG TTTGAGGCTG CAGTGAGCTA TGATCGTGCC ACTGCACTCC AGCCTGGGCA ACAGAGTGAG GCCCTGTCTT GAAAAAAAAA AAAAAAAGCA GCCACATGTG GCTAGTGGCT ACTATCTTGG ACAGTGCAGT CCTAGAAGAC ACAGGGGACT AGCTTTACGA CAACAGGACA TAAAAGTTAG GTTAGGGTTA AGATGGATAA ATCAGACTTG GTGCTGTGAG GCCTGGGCCC CGCTCTCCCT GATGCTAGCC CTCCCCTGAC TTGGACCATT AGGTCAAGTT CCTGTCTGCT TCGTCTCCGC AGAGCTGAGG AACTGCGTGT GGAGTCAGCC CAGTCTGGAT GCACAGGAGG ATGCTGGCGG CACAGTGAGT GAGGCCTGGT GCCAGAGCTG TGCGGACCCC TTGTTGGCCA TGGAGCAGCA GGCCCAGAGG CCCTCTCCCC AGCCCTGCTT GCCTGCCTCG GAGAGGACAG AGGCCTAGGC CCACGGGGGA GGGTGTTGGC AGACAGATGC CCTCCAGGCC CTGGGGCCTC CTTAACGGCC CCTTAACGAC ACGCGTGCCA AGGGTGGAGG CCTCATCATC ATTGCCTTCA GCCAGGGGGT GAGTGAGGGC AGCCCCTGGG CTTCACGTCT CGGCCCCAAC CTTGGCCAAG CTGCTATCTT CTCTTAGCCT CTTCTGTAAA ATGCTTATCT TCTGTAAAAT GCTATGCTTT TTTGTTTGTT TTGTTTTGTT TTTGGGTTTT TTTGAGACAG AGTCTCACTC TGTCACCCAG GCTGGAGTAC AGTGGCATGA TCTCGGCTCA CTGCAACCTC CATCTCCTGG GCTCAAGCAA TTCTCCTGCC TCAGCCTTCC GAGTAGCTAG GATGACAGGT GCATGCCACC ATGCCCAGCT AATTTTTGTA TTTTTAGTAG AGATGGGCTT TCACCATGTT GGCCAGGCTG GTCTCGAACT CCCAACCTCA GGTGATCCAC CTGCTTCGGC CCCCCAAAGT GCTGGGATTA TAGACGTGAG CCACCATGCC CGGCCAATGC TCTGTCTTTT ACAGCACATT TAGACTGGTA GAAGACAAGT TTCTAATTTA AAAAAATTTT TTTTGAGACT GGGCCTTGTT TTGTTGCCCA GGTTGGCCTT GAACTCCTGG GTTCAAGTAA TCATCCTGGC TTATCCTCCT

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001114, NM_001286057
• UniProt ID: P51828
• Synonyms: AC7
• Summary: This gene encodes a membrane-bound adenylate cyclase that catalyses the formation of cyclic AMP from ATP and is inhibitable by calcium. The product of this gene is a member of the adenylyl cyclase class-4/guanylyl cyclase enzyme family that is characterized by the presence of twelve membrane-spanning domains in its sequences. Several transcript variants have been observed for this gene, but the full-length natures of only two have been determined so far. [provided by RefSeq, Oct 2013]