NY-ESO-1 (CTAG1B) Human Gene Knockout Kit (CRISPR)

CAT#: KN213318

CTAG1B - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: NY-ESO-1
• Locus ID: 1485
• Components:

  KN213318G1, NY-ESO-1 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: TGGCATTCCTGATGGCCCAG

  KN213318G2, NY-ESO-1 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: CGGGGCACAGGGGGTTCGAC

  KN213318D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  ACCTGGCGCC TGGGAGACCT TGGCTGGCGC GAGGCCACGC CCACCAGACA TGCAGTTCCA GCTACCCCAC CAGCTGGGCG ACCAGGACAG GGACGGAGGC TGCTGAGCCC AGTTAGAGGC CTGCCCCCCG GGGTCTGTCC TGGGCGCTCC CCCAAGGACG GACAGGGCAG GCAGGGTCCG GGACGATGGC CGCACAGTCC CGGCCCCGTG TTCCCAGGCC CGTCTTGCTC CTCGATGTGA GGGAGACCCG GGGGATGGGA CAGGCTGGGC CCCGCAGTGC CTGACTCCCT GCAGGGCTCC CGGGACAGGG GTCCGGCGGA CAGCCGGCTG CTCAGGGGTG AGGGGTCCAA GCTGGCATTG CGGCCACCTT CCGGCCCGGG CTCTCTTGGG GAGGGGCGGG GTTGGTGAGA ACCGGTCACG TGCTCCGGGG CTCACTCGGG GTCTCCCAGG GCCGGAAGTA GGGCCCCTGT GCGCAGGCGC CCTGAGGATC CCGGGCTGCC CATCTCACGC CAGGGGGCGG AACTTCCTGC AGCCTCTCTG CCTCCGCATC CTCGTGGGCC CTGACCTTCT CTCTGAGAGC CGGGCAGAGG CTCCGGAGCC CAGGAGAGGC GGGTGCCACG GGCGGCAGAG GTCCCCGGGG CGCAGGGGCA GCAAGGGCCT CGGGGCCGGG AGGAGGCGCC CCGCGGGGTC CGCATGGCGG CGCGGCTTCA GGGCTGAATG GATGCTGCAG ATGCGGGGCC AGGGGGCCGG AGAGCCGCCT GCTTGAGTTG TATTCTGTTC TGTTCTGTTC TGTTCTGTTC TGACAGTTCT GGTGGCGAGG TGGGGGCCGG GAGATGGGGA GGGCAGGGCC AGGTGGGGGA GGAGGCGGGG GAGATGCGAG TAAGTGGTTG GGTGGGGGTG GGGGTGGGGG TTGGGAGGTG GGGATATGAG AGGCCAGCTG CGAGGAGGGG AGGAGGTAAG GGCTGGGAGG TAGAGGGGGT GGGGGTGGGG GAGAGGGGCT GGGTGATTGG GTGAGGGGCA CCGGGTCATG CGGGGAGGGG AGCAGGGGCC AGGAGATAGG GGGACCCAGG TGTAGGAGGG GATCAGGAGG TGGGGGGAAG GGGGCAAGAT GGGAGCGGGT GGCGGGAGGT GGTTGGGATG GAGGGTCGAG TGATGAGGAG GGGGCCGGGT GATGCCACAG GGGGCAGGGT GGGGAGGGGT

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001327
• UniProt ID: P78358
• Synonyms: CT6.1; CTAG; CTAG1; ESO1; LAGE-2; LAGE2B; NY-ESO-1
• Summary: The protein encoded by this gene is an antigen that is overexpressed in many cancers but that is also expressed in normal testis. This gene is found in a duplicated region of the X-chromosome and therefore has a neighboring gene of identical sequence. [provided by RefSeq, Jan 2012]