OGR1 (GPR68) Human Gene Knockout Kit (CRISPR)

CAT#: KN212925

GPR68 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: OGR1
• Locus ID: 8111
• Components:

  KN212925G1, OGR1 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GGTGGTCTATGTTACCGTGC

  KN212925G2, OGR1 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GTCGATGGTACAGCTCATCG

  KN212925D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  CCAGCTAATT TTTGTATTTT TAGTAGAGAC AGGGTTTTGC CATGTTGGCC AGGATGGTCT CGAACTCCTG ACCTCAAGTG ATCTGCCCAC CTTGGCCTCC CAAAGTGCTG GGAATACAGG TGTGAGCCAC CTCGCCTGGG GCTCCCATTC AGATCTATTC TTCTTGTTAT TTATTTATTT ATTTATTTAT TTATTTATTT ATTTATTTAT TTATAGAGAC AAGGGCTTGC CATGTTACCC AGGCTGGTCT TGAACTCTTG AGCTCGAGCG ATCCCCACCT CCCAAAGTGC TGGGCTTACA GGTGTAAGCC ATCATGTCCA GCCGTTCAGA TATTCTAGTT GAATTGGAGT TGGTGGGCTA GTACACCTTC TAAATTAAAT GAGTAAAGGA TTTAGAATGG TGCCTGACAC ACAGTAGGTG CTACATTCAT GTTAGCTACT ATTATAAACC TTTCCTGCCT CTGACTTTCA GGGTCTTGCC CACCACCAGC GATGCCCAGC CCTTGGTAGA GCTTGAACCA CCTTCTATAA ACAGGATGGC GGTGGAGAGA CAGGCCCAGT CCCTGAGCCC ATGAGGAGTG TGGCCCCTTC AGGCCCAAAG TGGTGGTGGG CTTCCCGGCC AACTGCCTGT CCCTCTACTT CGGCTACCTG CAGATCAAGG CCCGGAACGA GCTGGGCGTG TACCTGTGCA ACCTGACGGT GGCCGACCTC TTCTACATCT GCTCGCTGCC CTTCTGGCTG CAGTACGTGC TGCAGCACGA CAACTGGTCT CACGGCGACC TGTCCTGCCA GGTGTGCGGC ATCCTCCTGT ACGAGAACAT CTACATCAGC GTGGGCTTCC TCTGCTGCAT CTCCGTGGAC CGCTACCTGG CTGTGGCCCA TCCCTTCCGC TTCCACCAGT TCCGGACCCT GAAGGCGGCC GTCGGCGTCA GCGTGGTCAT CTGGGCCAAG GAGCTGCTGA CCAGCATCTA CTTCCTGATG CACGAGGAGG TCATCGAGGA CGAGAACCAG CACCGCGTGT GCTTTGAGCA CTACCCCATC CAGGCATGGC AGCGCGCCAT CAACTACTAC CGCTTCCTGG TGGGCTTCCT CTTCCCCATC TGCCTGCTGC TGGCGTCCTA CCAGGGCATC CTGCGCGCCG TGCGCCGGAG CCACGGCACC CAGAAGAGCC GCAAGGACCA GATCCAGCGG CTGGTGCTCA

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001177676, NM_003485, NM_001348437
• UniProt ID: Q15743
• Synonyms: GPR12A; OGR1
• Summary: The protein encoded by this gene is a G protein-coupled receptor for sphingosylphosphorylcholine. The encoded protein is a proton-sensing receptor, inactive at pH 7.8 but active at pH 6.8. Mutations in this gene are a cause of amelogenesis imperfecta. [provided by RefSeq, Feb 2017]