Matriptase 2 (TMPRSS6) Human Gene Knockout Kit (CRISPR)

CAT#: KN212708

TMPRSS6 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: Matriptase 2
• Locus ID: 164656
• Components:

  KN212708G1, Matriptase 2 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GAGGCTCCCTTGCAAATGCG

  KN212708G2, Matriptase 2 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: CACCGGTGGGGCACGGAAGC

  KN212708D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  CAGGTGAAGT ACCCACGTGG TGTGGGCTGG AGGAAGAAAA CATGGGACAA TGGTTCCACA TCCCTGGGTG ACCTGAAAAT TAAGTGTGAG ATGTCTCATG AGGGCACGAA ATGAATATTA GTTTTTGTTC CCTTCCTCTG CCACAAGACT TTGAGAGCAG AAAGGTGAGA GAGACGGTAC TCTGTGAAGG AAGGCAGGTC CCCGGCCCAG CGCAGTGCCA GCTCAGGGGA TTCTGGGGCG GGGGCTAAGT GCATGGACTG TGTGGGCGTG GTGGGAAGCT CCGTGAACCA GAACCAGGAG CAAGAAACAG CATTCCTTGC GTGGACGGGA AATGAGGGCA AGAGGTCAGA TGTCTACAGA AGTCTGCACC CCATGTACTT CAGTTCTGTC TGTGGGTGCA GCCTCTAGGG AGGTGGGTGT TTAGGTACTG AGACCTCCGT CTGTCCTCTG ACCATAGGGA AGCCAGTGGG AAGCAAAGGT GGGGTTCTTG AGCCAGACCC AGTCCAGCTC TGGTGCCTGC CCTCTGGTGC GAGCTGACCT GAGATGCACT TCCCTCCTCT GTGAGCTGTC TCGGCACCCA CTTGCAGTCA CTGCCGCCTG CCGTGGCCGA GGCCCCCCAG GTGGCTGGCG GGCAGGGGGA CGGAGGTGAT GGCGAGGAAG CGGAGCCGGA GGGGATGTTC AAGGCCTGTG AGGACTCCAA GAGAAAAGCC CGGGGCTACC TCCGCCTGGT GCCCCTGTTT GTGCTGCTGG CCCTGCTCGT GCTGGCTTCG GCGGGGGTGC TACTCTGGTA TTTCCTAGGT AACGTTGTGG GACCGCCTGG GAGAGGCACC TGGGGAGGAC TTGGGGTGAC TGTAGCAGGC ACAGCAGGAC AGGACTGGGT TCCAGGCTCA GCCGTGCTTA GCATATTGCT GTGTGACCTT GGGCAAGTCA CTTCTGTTCT CTGGGTCTCC CTCCCTGTCC TTCCAGCTGG AGATGCTGTC AGACCCTGGC TCCAGGTCCT ATGGCTCGGG TCTGCTTCCT GCTTGGGCAA AGTGCCCCAA AGCTCCCCAC CAGGTGGGGA AAGTGGGCCC TCCTAGCACC CAGTTCTTGT GAGCCAGCCA GCCCACAGAG CATAAACATC GCCTTCCCTT GCCTGCAGTC CTCCTGGGTT GCCCCTGAGG CTTGGAGCCA ACCCAGCCCT AAAGAAGGAG GCCCAGAGGC

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001289000, NM_001289001, NM_153019, NM_153609
• UniProt ID: Q8IU80
• Synonyms: IRIDA
• Summary: The protein encoded by this gene is a type II transmembrane serine proteinase that is found attached to the cell surface. The encoded protein may be involved in matrix remodeling processes in the liver. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]