MKS1 Human Gene Knockout Kit (CRISPR)

SKU KN211884LP

MKS1 - human gene knockout kit via CRISPR, HDR mediated

Product Data

• Format: 2 gRNA vectors, 1 Luciferase-Puro donor, 1 scramble control
• Vector: Luciferase-Puro
• Target Symbol: MKS1
• Locus ID: 54903
• Components:

  KN211884G1, MKS1 gRNA vector 1 in pCas-Guide CRISPR vector

  KN211884G2, MKS1 gRNA vector 2 in pCas-Guide CRISPR vector

  KN211884LPD, donor DNA containing left and right homologous arms and Luciferase-Puro functional cassette.

  GE100003, scramble sequence in pCas-Guide vector

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001165927, NM_001321268, NM_001321269, NM_001330397, NM_017777
• UniProt ID: Q9NXB0
• Synonyms: BBS13; JBTS28; MES; MKS; POC12
• Summary: The protein encoded by this gene localizes to the basal body and is required for formation of the primary cilium in ciliated epithelial cells. Mutations in this gene result in Meckel syndrome type 1 and in Bardet-Biedl syndrome type 13. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]
• Shipping: Ambient