Orexin Receptor 1 (HCRTR1) Human Gene Knockout Kit (CRISPR)

CAT#: KN210381

HCRTR1 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: Orexin Receptor 1
• Locus ID: 3061
• Components:

  KN210381G1, Orexin Receptor 1 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: TGCCAGGGGGGACCCCCATC

  KN210381G2, Orexin Receptor 1 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: AACTCATCTTCATAGTCTGG

  KN210381D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  CTCCCAAATC TACGGTGTTT GGTGGGTTCG AACAGACCTG GCTTAAGAGC TTTGTGACCT TGAGCAAGAG ACTCAATCTC TCTGAGCTTC GGTCTCATCT GCAAAGCAGG GTACCCTAAT AATGGTAACC GGAAACGTCC CCGAAACTAC CTTCTCGTAC CAGGTTCTTG GTGAAGCACT TGGCACGCAT CGGAGCTCAT TACTCCTCAT CGTGGTCCTG TAAGGTATGT AGGGCTGTCA CCCCATTAGA CAGATGGGGA AACCAAGGCT GAAAGAGGCC AGGTAAGCTA CCCAAGGCAA CTGGTGTGGA ATTGGGATGC AACCCAGGTC TGTCTTCCTC CACCAATTTC ATGACTGTGA GAATTAAGAG GGAACTTATA CGCAAAGCGC CTGGCACAAT CCCTAATGTT TCCTTCCTTC TCTCTTTTCC CACTCCCTCC TTTCCTTCCT CCCTTCAGGA AGTTTGAGGC TGAGACCCGA AAAGACCTGG GTGCAAGCCT CCAGGCACCC TGAAGGGAGT GGGCTGAGGG CTGGCCCAAG CTCCCTCCTC TCCCTCTGTA GAGCCTAGGA TGCCCCTCTG CTGCAGCGGC TCCTGAGCTC TCTCCGCTAT CTGTGGCGCG ATTATCTGTA CCCAAAACAG TATGAGTGGG TCCTCATCGC AGCCTATGTG GCTGTGTTCG TCGTGGCCCT GGTGGGCAAC ACGCTGGGTA GGTCCAGGGC TTGCCCGGCA GTGCTGCCGG CTTTCCCTGG GGATTGAAGG GGGTTGTGTG GGAGGAGGGC TCGCTGATTA GGCAGAACTA GGATGGGTGT GGCTCTGCCA CCAGCTTCAC CTCGCTGCAC CCTGCAGTCT GCCTGGCCGT GTGGCGGAAC CACCACATGA GGACAGTCAC CAACTACTTC ATTGTCAACC TGTCCCTGGC TGACGTTCTG GTGACTGCTA TCTGCCTGCC GGCCAGCCTG CTGGTGGACA TCACTGAGTC CTGGCTGTTC GGCCATGCCC TCTGCAAGGT CATCCCCTAT CTACAGGTGA GCTCTGCCCA GGCACCCCTC ACCACTCCTT GTCACGCCTG TAAAAAACCC ACGGCCTTGC ATAGGTCTCA GTGACCCCCA GACTTGCCTT TCAGACAGGT CAGTGGCTCA TGACCCCTGA AGTGTCATCC TCTGCTGCTA GCAAGGGCAA GCCACCAGAT CAGACACTCG

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001525
• UniProt ID: O43613
• Synonyms: OX1R
• Summary: The protein encoded by this gene is a G-protein coupled receptor involved in the regulation of feeding behavior. The encoded protein selectively binds the hypothalamic neuropeptide orexin A. A related gene (HCRTR2) encodes a G-protein coupled receptor that binds orexin A and orexin B. [provided by RefSeq, Jan 2009]