MIRO1 (RHOT1) Human Gene Knockout Kit (CRISPR)

CAT#: KN209190

RHOT1 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: MIRO1
• Locus ID: 55288
• Components:

  KN209190G1, MIRO1 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GAACGTGAGTCCGCACCCTC

  KN209190G2, MIRO1 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GAAAGACGTGCGGATCCTGC

  KN209190D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  TTTTCTGACG GCCCTATCCA ATAACGTGGG GACGCAGCTG AAAACCATGC AAGCTGATCG CTGCCCTCCC GGAGTTTATG GTCTGGTGAG AAGATCCCTG CTCTGAGTCA CTGCCAAGCT CTAGCTATGT GACTTTGAAC AGGGAATTTC TGTCCCTATC TATAAAATAA ACGGGGTTAA ACCAGGGAAT CCGCCATTCA AATTCCTGGC CCAAGTGTCT CCGGGGTCGC GGTGCCCAAA GGAGGGTGGG CGAGGCTCCC TGGGCCTGCG CTGCGGCGGC CTGACAGCGG GAGCGCTGTT CCGGCGCAGA GCGCGTTCGC GTTCCGAAAG CGCGTGTCCC TCGCCGCGTG CCGTCGTCCC CGCCGCCGCC GCCGCCGCCG CCGCCGCCGC CGCCGCCGCC GCCGCCGCCA CAGCCCGCTG GGCCGGAGGA GGCGGAGCTG GCGCTGTCCC GGCTCTCTTG CGGGGAAGCA ACTGAGGGGG CGGCGCGGCG GGCCCCGGCG GCCGAAGAGG CTGGCAGGTG GCGCCGTGGG GTGGGTGCTC CTGGTGAGAG GAGTCCACTC CGTGCGTGCG GGCGGAGGCC GGCCCCCGAG AGCCGCCGAC TCCCTGCCCT CCCTGCCCCT GCAGCCCCTG TCGCCCCTGT CGCCCCTGCA GCCCCAACCT TTGCAGCCCC GGCCCTTCTC GCGCCTCACA GCTCCGCTCG CCTTTCTCCG CGTTCCTAGG CCTTCCAGCC CCTTCACTCT TCTCTTTTGG CTGGCCGCCG GCGGCCCTCA CCCTGTCACC TCACCTGGGC CCTCCAGAGA TCTCCTTTCC CTGTTCCCCA GCCGTCCTCC CAAGCCATGT CCCTATTAGC CCTAACCGCC CGACCTCATC CCTCCGAAGC TTCTCTCCTC CTTTTCCTCT TCGAACCCTG TCCTTCTGGG CCTCACAGTT TCCCAGGCCG CCTCCTTTCA GACCTTCTTG TCTTCTGGAG ATTCCCTTAT CACTGCCAGG CAGGTGCTGG AATAAGCAGA CTGACCAATA GGGATTGCGT GAACCGGCTC CCGGGGAAGC TGGGGGAGGG GTGCAGAACT ATTCGACTTG TGGGCAAGGT CAGGTTTCTG CATTTTTTTG GCACTCGCAG TCTTCACTCT CTCTATGTAT TGACATTAAG ATATGAGTAG TGGAAAGGGC CGGATCCTTT TTAGCAGTAC TTGTACTTTG

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001033566, NM_001033567, NM_001033568, NM_001288754, NM_001288755, NM_001288758, NM_018307, NR_110083
• UniProt ID: Q8IXI2
• Synonyms: ARHT1; MIRO-1; MIRO1
• Summary: Mitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution.[UniProtKB/Swiss-Prot Function]