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MIRO1 (RHOT1) Human Gene Knockout Kit (CRISPR)

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SKU
KN209190
RHOT1 - human gene knockout kit via CRISPR, HDR mediated
  $1,657.00
4 Weeks*

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Specifications
Specifications
Product Data
Format 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Vector GFP-puro
Target Symbol MIRO1
Locus ID 55288
Components

KN209190G1, MIRO1 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GAACGTGAGTCCGCACCCTC

KN209190G2, MIRO1 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GAAAGACGTGCGGATCCTGC

KN209190D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

Homologous arm and GFP-puro sequences:

pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

TTTTCTGACG GCCCTATCCA ATAACGTGGG GACGCAGCTG AAAACCATGC AAGCTGATCG CTGCCCTCCC GGAGTTTATG GTCTGGTGAG AAGATCCCTG CTCTGAGTCA CTGCCAAGCT CTAGCTATGT GACTTTGAAC AGGGAATTTC TGTCCCTATC TATAAAATAA ACGGGGTTAA ACCAGGGAAT CCGCCATTCA AATTCCTGGC CCAAGTGTCT CCGGGGTCGC GGTGCCCAAA GGAGGGTGGG CGAGGCTCCC TGGGCCTGCG CTGCGGCGGC CTGACAGCGG GAGCGCTGTT CCGGCGCAGA GCGCGTTCGC GTTCCGAAAG CGCGTGTCCC TCGCCGCGTG CCGTCGTCCC CGCCGCCGCC GCCGCCGCCG CCGCCGCCGC CGCCGCCGCC GCCGCCGCCA CAGCCCGCTG GGCCGGAGGA GGCGGAGCTG GCGCTGTCCC GGCTCTCTTG CGGGGAAGCA ACTGAGGGGG CGGCGCGGCG GGCCCCGGCG GCCGAAGAGG CTGGCAGGTG GCGCCGTGGG GTGGGTGCTC CTGGTGAGAG GAGTCCACTC CGTGCGTGCG GGCGGAGGCC GGCCCCCGAG AGCCGCCGAC TCCCTGCCCT CCCTGCCCCT GCAGCCCCTG TCGCCCCTGT CGCCCCTGCA GCCCCAACCT TTGCAGCCCC GGCCCTTCTC GCGCCTCACA GCTCCGCTCG CCTTTCTCCG CGTTCCTAGG CCTTCCAGCC CCTTCACTCT TCTCTTTTGG CTGGCCGCCG GCGGCCCTCA CCCTGTCACC TCACCTGGGC CCTCCAGAGA TCTCCTTTCC CTGTTCCCCA GCCGTCCTCC CAAGCCATGT CCCTATTAGC CCTAACCGCC CGACCTCATC CCTCCGAAGC TTCTCTCCTC CTTTTCCTCT TCGAACCCTG TCCTTCTGGG CCTCACAGTT TCCCAGGCCG CCTCCTTTCA GACCTTCTTG TCTTCTGGAG ATTCCCTTAT CACTGCCAGG CAGGTGCTGG AATAAGCAGA CTGACCAATA GGGATTGCGT GAACCGGCTC CCGGGGAAGC TGGGGGAGGG GTGCAGAACT ATTCGACTTG TGGGCAAGGT CAGGTTTCTG CATTTTTTTG GCACTCGCAG TCTTCACTCT CTCTATGTAT TGACATTAAG ATATGAGTAG TGGAAAGGGC CGGATCCTTT TTAGCAGTAC TTGTACTTTG

GE100003, scramble sequence in pCas-Guide vector
OTI Disclaimer These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq NM_001033566, NM_001033567, NM_001033568, NM_001288754, NM_001288755, NM_001288758, NM_018307, NR_110083
UniProt ID Q8IXI2
Synonyms ARHT1; MIRO-1; MIRO1
Summary Mitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution.UniProtKB/Swiss-Prot Function
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Documents
Resources
Other Versions
SKU Description Size Price
GA110676 RHOT1 CRISPRa kit - CRISPR gene activation of human ras homolog family member T1 1 kit
$1,657.00
KN209190BN RHOT1 - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN209190LP RHOT1 - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN209190RB RHOT1 - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN409190 RHOT1 - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated. 1 kit
$1,657.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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