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CD146 (MCAM) Human Gene Knockout Kit (CRISPR)

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SKU
KN208937
MCAM - human gene knockout kit via CRISPR, HDR mediated
  $1,657.00
4 Weeks*

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Other products for "CD146 or NM_006500"

Specifications
Specifications
Product Data
Format 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Vector GFP-puro
Target Symbol CD146
Locus ID 4162
Components

KN208937G1, CD146 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GGGTGAGTTCGCTCGCAGGG

KN208937G2, CD146 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: CCACCGCAGACCCCTAGCCG

KN208937D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

Homologous arm and GFP-puro sequences:

pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

TCTGTGGGAC TGGCGCAGGG TTCTTCTCCC AAAGCTGCCA GCACTGAGGC TGAGGCAGTG TCAGGCCGGC GGCAGCGGCA GTGGTGCAAT CGTTCTGGGA AGCGATAGAG GCCGGCCTGA ATTCTCTGTG GCAAGGGAGG GGAGCCCAAG TGGGAGGCCC CTTGGGGACA CCGAGGACCA GGTCCGCTAC TGCTCCTCCC CCAGGAGGTC CCCTAGGGGC TACATTGGCT GGCAGGGGCT GAGCAGCGGT GAGCCTGGCT GGCTTCGACC CGGGGCGACT CCGGGCATCC GGGACAGCTT CTCCTCGCTG CCACCTCGGC CAGTCAGACC CCGAGACACC TGTCACTACC CCCTCAGCCT TCCCAAGCCA GGAGCCTGGG AGTCCGGCTC TGGCCTACCT CCGGCAGCGC TCCTAGGCGC AGCTCCCGGG CTGGCGGCGC CGGGGCCCGC CCCCTAGGGC TGCGGCGCGC GGGGCGGGGG CTGGGGGCTG CGCGGGGCGG GGCGGGCCCG GGCGCTCCGG GCCCCCTCCC CCGCCCCCCT GACGTCAGCC CCCGGCAGCC TCGAGCTGCT CACTTGGCTC TCGCCCTCCG GCCGGGAAGC GCGCGGCGGG GTCGGGGCGT TGCGTCTGGG AGGACGAGCC TCCTCCCTGG GTCCCCGATC CCCGGGCGCC TTGCGCGCGA GCAACTCTTC TTTGCAGCCA GTTTGCAGCC GGGATTCTAG AGTATCCCGG GAGCAGCACT CGGAAGGCGG GGAGGAGGCT GCTTCTGGGA ACGAGAAGGG GTGGAGCTCA GCCTTTCGGG GTGCTGGGGG GTGGGTGGTC CCTGAGGTGC TCACTCTGGG GGCCCGCAAT TGAAGCCGGG CAGGAGGCGC AGCTGGGGCG CATCCTCAAA GCCTGAATTC CGCGCCCGCC TGTTGCTGGA AAAGGCAGCT TCCTTCGCTG GAGGGGGTGC GCCGACCCAC CCCTTCCCCC TTCTGCCTGG GCATCACGCC AGGCTGGAGG TGAGCGAGAG CGGGAGGTTC GGCGGCTCCC GCCCGAGCTG GGCGTTGGCA GGGGTTGCGG GGCGGTGTGG GTCGCCTCGC GCCTCCCCGA GTGATGGGAT CATAGGGGAC AGAGATGAGG GATGGAGGAT TCCCATACTG GAAGCGCCCG CTGGCTTATT TTGGGGACCA CATTCAGGTG GGAAGTGCGC CCGGGCACCT

GE100003, scramble sequence in pCas-Guide vector
OTI Disclaimer These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq NM_006500
UniProt ID P43121
Synonyms CD146; MUC18
Summary Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.UniProtKB/Swiss-Prot Function
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Documents
Resources
Other Versions
SKU Description Size Price
GA102839 MCAM CRISPRa kit - CRISPR gene activation of human melanoma cell adhesion molecule 1 kit
$1,657.00
KN208937BN MCAM - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN208937LP MCAM - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN208937RB MCAM - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN408937 MCAM - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated. 1 kit
$1,657.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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