Oct-1 (POU2F1) Human Gene Knockout Kit (CRISPR)

CAT#: KN208599

POU2F1 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: Oct-1
• Locus ID: 5451
• Components:

  KN208599G1, Oct-1 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: ATAAATTATGCTAAGTCGCG

  KN208599G2, Oct-1 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: AGATGAGAGTTCAGCCGCGG

  KN208599D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  TTGCCAATGT TTCAACTGCG CGGAAACCCT GAAGAAAGTG CGGAGCACAG GGCACGCGGG CCGCTCACTG CCCTTCCACG CGCCCCCACG TTTCTGGCCG CTGCGCTCCT TGCCGTGCTT TTACAATGCC ACAGGCCACT GGGGGAGACC GGAGCGAAGC CAGGAGGGAA GCAAGGCGGG AGGAAAAGGG AGAAGTGTAA GAAGACACAG GCGCCCGGGA TACTATATAA TCTGGTTCTG AGCTAGAGGG AGCGCATGCG CCGGCGACAC TTCTGGCGGC AGGAGAAAGG CGCGCATGCG CAGAGCCGAG ACGAAAAATG AAGCCACAGG GTGCCTGGCT GATTGAGGGC GCTGCTTTAG CTACCGAAGG GCCACAGCGC CCCGAAGGCG CGTGCGCAGA GCGCGGGAAG GGGGTGGAGC TCTTCTGAGG GGGCCGGGGC GAACAGAGCC GGCCTCGCGC GCCCCCTGGC GCGCGCCCGG GGAGAGGAAG GAGCGGGAGG GGGCGGAGAG GAGGGAGGAG GCAAGCAGGG CGGGAGGGGG GAGGGGAGCC AGAGCGAGGG AGGGTTTATC GACCGGGCGA TTTTGGTTAA AATATTCAAA ACTCAGGATT ATTATAATTA ACGGCGGGGA GATGGGGGGC CGGGGAGCAT TGAGCCCCCG CCGGGCGCTG AGCGGAGGGG GAAAGAGGCT CGGAGCGGCC AGGCGGAGGG GGAAGACAAG GGGCAAAGGG GGCTGCGATC CACTGCCCTC CTCCTGCACC CGGCCCACCC CGCCGCCGCT CGCCGTCTGC CCGTCGGCAC CGGCCCTCCT CCTCTGCCGG CCGCCGCTGC CGCCTCCTCG CCGCCGTGCC CGAACCCGAG CGGGTCCGCG GCGGGGCGGC GGGGCCGGGG CAGAGGGAGA GGACAATAAA GACATTTTAC ATATTCATAG CTGCTGGGCC GGCGGGGCGC AGTAGGGCGC GGGCGGGCGG CCGGGGCCGG GCGGGCGCGG GGTGGGGGGC GTGAAGGGGG ATGGAGGCGG CGGCGAGCAC AATGCCGGGG AGGCGGCGGG GCCGGAGCGG ACCCCGGCGG CCGGACACCC GCACGCGTCT TCCCCCGGGA GCGGGCCCGG GCGGGGGGGC GCTGCCCGCC TTGGGGGGCG GCCCTCAGGG CAACCGGGGG TGCGCGCGGG GAGAGCTCCC TGCCCGCGCC CCGCGCGGGG

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001198783, NM_001198786, NM_002697, NR_037163, NM_001365848, NM_001365849
• UniProt ID: P14859
• Synonyms: oct-1B; OCT1; OTF1
• Summary: The OCT1 transcription factor was among the first identified members of the POU transcription factor family (summarized by Sturm et al., 1993 [PubMed 8314572]). Members of this family contain the POU domain, a 160-amino acid region necessary for DNA binding to the octameric sequence ATGCAAAT.[supplied by OMIM, Jul 2010]