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Oct-1 (POU2F1) Human Gene Knockout Kit (CRISPR)

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SKU
KN208599
POU2F1 - human gene knockout kit via CRISPR, HDR mediated
  $1,657.00
4 Weeks*

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Specifications
Specifications
Product Data
Format 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Vector GFP-puro
Target Symbol Oct-1
Locus ID 5451
Components

KN208599G1, Oct-1 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: ATAAATTATGCTAAGTCGCG

KN208599G2, Oct-1 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: AGATGAGAGTTCAGCCGCGG

KN208599D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

Homologous arm and GFP-puro sequences:

pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

TTGCCAATGT TTCAACTGCG CGGAAACCCT GAAGAAAGTG CGGAGCACAG GGCACGCGGG CCGCTCACTG CCCTTCCACG CGCCCCCACG TTTCTGGCCG CTGCGCTCCT TGCCGTGCTT TTACAATGCC ACAGGCCACT GGGGGAGACC GGAGCGAAGC CAGGAGGGAA GCAAGGCGGG AGGAAAAGGG AGAAGTGTAA GAAGACACAG GCGCCCGGGA TACTATATAA TCTGGTTCTG AGCTAGAGGG AGCGCATGCG CCGGCGACAC TTCTGGCGGC AGGAGAAAGG CGCGCATGCG CAGAGCCGAG ACGAAAAATG AAGCCACAGG GTGCCTGGCT GATTGAGGGC GCTGCTTTAG CTACCGAAGG GCCACAGCGC CCCGAAGGCG CGTGCGCAGA GCGCGGGAAG GGGGTGGAGC TCTTCTGAGG GGGCCGGGGC GAACAGAGCC GGCCTCGCGC GCCCCCTGGC GCGCGCCCGG GGAGAGGAAG GAGCGGGAGG GGGCGGAGAG GAGGGAGGAG GCAAGCAGGG CGGGAGGGGG GAGGGGAGCC AGAGCGAGGG AGGGTTTATC GACCGGGCGA TTTTGGTTAA AATATTCAAA ACTCAGGATT ATTATAATTA ACGGCGGGGA GATGGGGGGC CGGGGAGCAT TGAGCCCCCG CCGGGCGCTG AGCGGAGGGG GAAAGAGGCT CGGAGCGGCC AGGCGGAGGG GGAAGACAAG GGGCAAAGGG GGCTGCGATC CACTGCCCTC CTCCTGCACC CGGCCCACCC CGCCGCCGCT CGCCGTCTGC CCGTCGGCAC CGGCCCTCCT CCTCTGCCGG CCGCCGCTGC CGCCTCCTCG CCGCCGTGCC CGAACCCGAG CGGGTCCGCG GCGGGGCGGC GGGGCCGGGG CAGAGGGAGA GGACAATAAA GACATTTTAC ATATTCATAG CTGCTGGGCC GGCGGGGCGC AGTAGGGCGC GGGCGGGCGG CCGGGGCCGG GCGGGCGCGG GGTGGGGGGC GTGAAGGGGG ATGGAGGCGG CGGCGAGCAC AATGCCGGGG AGGCGGCGGG GCCGGAGCGG ACCCCGGCGG CCGGACACCC GCACGCGTCT TCCCCCGGGA GCGGGCCCGG GCGGGGGGGC GCTGCCCGCC TTGGGGGGCG GCCCTCAGGG CAACCGGGGG TGCGCGCGGG GAGAGCTCCC TGCCCGCGCC CCGCGCGGGG

GE100003, scramble sequence in pCas-Guide vector
OTI Disclaimer These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq NM_001198783, NM_001198786, NM_002697, NR_037163, NM_001365848, NM_001365849
UniProt ID P14859
Synonyms oct-1B; OCT1; OTF1
Summary The OCT1 transcription factor was among the first identified members of the POU transcription factor family (summarized by Sturm et al., 1993 PubMed 8314572). Members of this family contain the POU domain, a 160-amino acid region necessary for DNA binding to the octameric sequence ATGCAAAT.supplied by OMIM, Jul 2010
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Documents
Resources
Other Versions
SKU Description Size Price
GA103673 POU2F1 CRISPRa kit - CRISPR gene activation of human POU class 2 homeobox 1 1 kit
$1,657.00
KN208599BN POU2F1 - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN208599LP POU2F1 - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN208599RB POU2F1 - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN408599 POU2F1 - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated. 1 kit
$1,657.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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