YES1 Human Gene Knockout Kit (CRISPR)

CAT#: KN208432

YES1 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: YES1
• Locus ID: 7525
• Components:

  KN208432G1, YES1 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: AGTACAAGTGTGAGCCATTA

  KN208432G2, YES1 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: TCAGGTCTGTATTTAATGGC

  KN208432D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  GGGTTCACGC CATTCTCCTG CCTCAGCCTC CCGAGTAGCT GGGACTACAG GCGCCCACCA CCACGCCCGG CTAATTTTTT GCATTTTTAG TAGAGGCGGG GTTTCACCGT GTTAGCCAGG ATGGTCTCAA TCTCCTGACC TCGTGATCCG CCCGCCTTGG CCTCCCAAAG TGCTGGGATT ACAGGCGTGA GCCACTGCGC CCAGCGATAC TTTTTCAAGT AACTGGAGTG CATAGAGGTT AAGCAACATA CCCTGAAATC ACTCCACTAC TAAGTAGGAA GCCAGGATTC AAATTTAGGC CTCTCTGGCT TCAGAGCCCA TGGTTTTTTC CCAATATACT GTGGCATGTT GTCATTGGAC TATTAAAGGT GATTGAGTTG GAGAATGAAG CGATTGGAGT AGGGCTTTTG CAAATTGTAA TAAAGATAAA TGAGGGGAAG AGACCTCGTT TTCAGCTTTC TATTTTTTGT TTTGTTTATG TTCCTTTTTG CAGGGATGGC ATATGCTTTT CTTAAAATGA TTTTTTGAAC CCTGTCTTTT TGAAGTATCC TGTGTGTTAA CTGACTCTCA AAATATTTTA TTGTCTCTGT AGATTTGATA CAGAACCCAC TACAGTGTCA CCATGTCCGT CATCTTCAGC AAAGGGAACA GCAGTTAATT TCAGCAGTCT TTCCATGACA CCATTTGGAG GATCCTCAGG GGTAACGCCT TTTGGAGGTG CATCTTCCTC ATTTTCAGTG GTGCCAAGTT CATATCCTGC TGGTTTAACA GGTGAGATTT AAATGGACAA TTATGTTGTC TGTTTGAGAA CATCACCTTG GGTAATATAT ACTTAAGGCT TGTCTGCCTT TAAGAAAGAT ATCTTACTTG AGGACTTTTC AAATAATTGT ATGTGAAAAT CAGTCTCTAA CATAAAAATT AGTTTTATTG TATACATGGG TATGGTTCTG GAAAATTGGT AGTAACCTGA TGGTGATTAT GTACTGGATC ATGTCATTAA TTATTTTATT AACTCATTAT TCAAATAGTT ATTGAGCCCC CCCCCACTGT AGGCACCGGG AACATAAAGA TTAAAAACAT ATACCTTTCC CTCAAGAATT TGACAACTTC TCAGGATTGT AAAGTACTTT GCAGTATTAA AGTCTTATAG TGGTTACTTA ATAGATGTTA ATTAAATGAC AGTAGAGTAG CGATAATACA

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_005433
• UniProt ID: P07947
• Synonyms: c-yes; HsT441; P61-YES; Yes
• Summary: This gene is the cellular homolog of the Yamaguchi sarcoma virus oncogene. The encoded protein has tyrosine kinase activity and belongs to the src family of proteins. This gene lies in close proximity to thymidylate synthase gene on chromosome 18, and a corresponding pseudogene has been found on chromosome 22. [provided by RefSeq, Jul 2008]