JNK2 (MAPK9) Human Gene Knockout Kit (CRISPR)

SKU KN207608BN

MAPK9 - human gene knockout kit via CRISPR, HDR mediated

Product Data

• Format: 2 gRNA vectors, 1 mBFP-Neo donor, 1 scramble control
• Vector: mBFP-Neo
• Target Symbol: JNK2
• Locus ID: 5601
• Components:

  KN207608G1, JNK2 gRNA vector 1 in pCas-Guide CRISPR vector

  KN207608G2, JNK2 gRNA vector 2 in pCas-Guide CRISPR vector

  KN207608BND, donor DNA containing left and right homologous arms and mBFP-Neo functional cassette.

  Homologous arm and mBFP-Neo sequences:

  pUC vector backbone in gray; Left arm sequence in blue; mBFP-Neo in green; Right arm in violet

  AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTCTAAG AAACCATTAT TATCATGACA TTAACCTATA AAAATAGGCG TATCACGAGG CCCTTTCGGG TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG TTGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATAAAAT TGTAAACGTT AATATTTTGT TAAAATTCGC GTTAAATTTT TGTTAAATCA GCTCATTTTT TAACCAATAG GCCGAAATCG GCAAAATCCC TTATAAATCA AAAGAATAGC CCGAGATAGG GTTGAGTGTT GTTCCAGTTT GGAACAAGAG TCCACTATTA AAGAACGTGG ACTCCAACGT CAAAGGGCGA AAAACCGTCT ATCAGGGCGA TGGCCCACTA CGTGAACCAT CACCCAAATC AAGTTTTTTG GGGTCGAGGT GCCGTAAAGC ACTAAATCGG AACCCTAAAG GGAGCCCCCG ATTTAGAGCT TGACGGGGAA AGCCGGCGAA CGTGGCGAGA AAGGAAGGGA AGAAAGCGAA AGGAGCGGGC GCTAGGGCGC TGGCAAGTGT AGCGGTCACG CTGCGCGTAA CCACCACACC CGCCGCGCTT AATGCGCCGC TACAGGGCGC GTACTATGGT TGCTTTGACG TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGCCATTC GCCATTCAGG CTGCGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG CCAGCTGGCG AAAGGGGGAT GTGCTGCAAG GCGATTAAGT TGGGTAACGC CAGGGTTTTC CCAGTCACGA CGTTGTAAAA CGACGGCCAG TGAATTGGAG GCTACAGTCA GTGGAGAGGA CTTTCACAGG CTGTCGCCGT GCTCATTTGA TAACTGCCCG TTATTCATGC GACACGCACA TGCAGCACAC TGATGCCACC AGGCCTCTGA CAGCCTGTCA CTTTGGCGTA TGAATCTTGC TTTTTGGTTC ATCATTTCAA CCAGAATCTA TACACTGAGC AGAAGACTGG TCCGTTTGCT TCTCTTTAGG CATTCTAGAA GTGATCATTT TTGAAACAAT GCCTCCGTCT CTAGACAGCT AGGTGTAAAT GAACCATTTA CACCTAGCTT GCCCACAGGG GGCAAGCTGA TGAGACGCAC AGAGTGATGT AGTCTTTTCA TAGCCAGTAG TAATGCATAC ATTCTTTTCT CCAAAGGTGG TTATTTTCTA GTCCAGCCCC TTAAAGTGTG GTCTTGCTGG GCCTCAAAAT TCCTTCCTTT CATAATGGTC TGGTACCACT GAAATGTCTG CATTTTTTTG TGTCTTGCTT AAGTTCAGAA CTTGGTCAGA TTGACTTTAT CAACTTACAG CCACATTGTC TTACATACCA ATAAGTTCAG AACTCTCTTA CGTGAGCTGC GTATGTACTT CCGGTAAGAA TGCACGTTCA TTCTGTTTTT CTTTGAAGGG ATCTGAAACT TGCCCACCCT TCGGGATATT GCAGGACGCT GCATCACTAG CATGAGCGAG CTGATTAAGG AGAACATGCA CATGAAGCTG TACATGGAGG GCACCGTGGA CAACCATCAC TTCAAGTGCA CATCCGAGGG CGAAGGCAAG CCCTACGAGG GCACCCAGAC CATGAGAATC AAGGTGGTCG AGGGCGGCCC TCTCCCCTTC GCCTTCGACA TCCTGGCTAC TAGCTTCCTC TACGGCAGCA AGACCTTCAT CAACCACACC CAGGGCATCC CCGACTTCTT CAAGCAGTCC TTCCCTGAGG GCTTCACATG GGAGAGAGTC ACCACATACG AAGACGGGGG CGTGCTGACC GCTACCCAGG ACACCAGCCT CCAGGACGGC TGCCTCATCT ACAACGTCAA GATCAGAGGG GTGAACTTCA CATCCAACGG CCCTGTGATG CAGAAGAAAA CACTCGGCTG GGAGGCCTTC ACCGAGACGC TGTACCCCGC TGACGGCGGC CTGGAAGGCA GAAACGACAT GGCCCTGAAG CTCGTGGGCG GGAGCCATCT GATCGCAAAC ATCAAGACCA CATATAGATC CAAGAAACCC GCTAAGAACC TCAAGATGCC TGGCGTCTAC TATGTGGACT ACAGACTGGA AAGAATCAAG GAGGCCAACA ACGAGACCTA CGTCGAGCAG CACGAGGTGG CAGTGGCCAG ATACTGCGAC CTCCCTAGCA AACTGGGGCA CTAAATCGAT CATATTCAAT AACCCTTAAT ATAACTTCGT ATAATGTATG CTATACGAAG TTATTAGGTC TGAAGAGGAG TTTACGTCCA GCCAAGCTTA GGATCTCGAC CTCGAAATTC TACCGGGTAG GGGAGGCGCT TTTCCCAAGG CAGTCTGGAG CATGCGCTTT AGCAGCCCCG CTGGGCACTT GGCGCTACAC AAGTGGCCTC TGGCCTCGCA CACATTCCAC ATCCACCGGT AGGCGCCAAC CGACTCCGTT CTTTGGTGGC CCCTTCGCGC CACCTTCTAC TCCTCCCCTA GTCAGGAAGT TCCCCCCCGC CCCGCAGCTC GCGTCGTGCA GGACGTGACA AATGGAAGTA GCACGTCTCA CTAGTCTCGT GCAGATGGAC AGCACCGCTG AGCAATGGAA GCGGGTAGGC CTTTGGGGCA GCGGCCAATA GCAGCTTTGC TCCTTCGCTT TCTGGGCTCA GAGGCTGGGA AGGGGTGGGT CCGGGGGCGG GCTCAGGGGC GGGCTCAGGG GCGGGGCGGG CGCCCGAAGG TCCTCCGGAG GCCCGGCATT CTGCACGCTT CAAAAGCGCA CGTCTGCCGC GCTGTTCTCC TCTTCCTCAT CTCCGGGCCT TTCGACCTGC ATCCATCTAG ATCTCGAGCA GCTGAAGCTT ACCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCCGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGACG CCCGCCCCAC GACCCGCAGC GCCCGACCGA AAGGAGCGCA CGACCCCATG CATCGATGAT ATCAGATCCC CGGGATGCAG AAATTGATGA TCTATTAAAC AATAAAGATG TCCACTAAAA TGGAAGTTTT TCCTGTCATA CTTTGTTAAG AAGGGTGAGA ACAGAGTACC TACATTTTGA ATGGAAGGAT TGGAGCTACG GGGGTGGGGG TGGGGTGGGA TTAGATAAAT GCCTGCTCTT TACTGAAGGC TCTTTACTAT TGCTTTATGA TAATGTTTCA TAGTTGGATA TCATAATTTA AACAAGCAAA ACCAAATTAA GGGCCAGCTC ATTCCTCCCA CTCATGATCT ATAGATCTAT AGATCTCTCG TGGGATCATT GTTTTTCTCT TGATTCCCAC TTTGTGGTTC TAAGTACTGT GGTTTCCAAA TGTGTCAGTT TCATAGCCTG AAGAACGAGA TCAGCAGCCT CTGTTCCACA TACACTTCAT TCTCAGTATT GTTTTGCCAA GTTCTAATTC CATCAGAAGC TGGTCGAGAT CCGGAACCCT TAATATAACT TCGTATAATG TATGCTATAC GAAGTTATTA GGTCCCTCGA AGAGGTTCAC TAGGCGCGCC AGCGTGGATT GAGTTTTTGC TGTAATAAAA AGTAAAACTA AAGTGTCAGG ATTTAGAAAC ATTGATGATT AAAAAAAAAA CTCTGTTATA GGTTTATGAG CTAAATTGAA TCAACTGCTT GCTCTAGTTC TGTGTAGTCA GGCTGTAGGG TACAAACCCA TATTCTTAGA ATGCTGCAGG AATTTAAATA GGTAGCAAGT TTTCTTTTTT GTTTGTTTCT TTTTCCTTTC ATTTTTTTTT TCATTTCTCC TGTCATTATG ATATCAGAGT AGTAGGTATG ACAGTGAGTT GTAACAGAAA TTTGGAGTTT TATTCATTTC TTCATCATGG CCACCTTTTC CCCCCTCCCC TTTTATTCCC GATCACTCCT GTTCATTTCC AGGCGCCATC ATTGCAGTGC CTTGCCATTC ATTGGCAGGA TGAGGCCAGT CAGTGTCATT GTCACTCCAT GGATTAACCA GGAAGGGATG CTGTGTGAGG GGTTGGGCTG GCCATCTCTT CCTTGGTGTC TCACTTCAAC TGTGTGTCCA GCCCAGAACG TCAGAATCGT TAGTCTGACC CTGAACGTTA GTCTCTTGCA TGGTAAGACG GTCAGCCGCC TCACTCTCGC CGGTTGGACT TTAGATCAGA AGGGATCTTG CTGCCGCCCG AAAGAGGAAG GGCTGGAAGA GGAAGGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGAAC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATC

  GE100003, scramble sequence in pCas-Guide vector

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001135044, NM_001308244, NM_002752, NM_139068, NM_139069, NM_139070, NM_001364610, NM_001364607, NM_001364608, NM_001364609, NM_001364612, NM_001364613, NM_001364611
• UniProt ID: P45984
• Synonyms: JNK-55; JNK2; JNK2A; JNK2ALPHA; JNK2B; JNK2BETA; p54a; p54aSAPK; PRKM9; SAPK; SAPK1a
• Summary: The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase targets specific transcription factors, and thus mediates immediate-early gene expression in response to various cell stimuli. It is most closely related to MAPK8, both of which are involved in UV radiation induced apoptosis, thought to be related to the cytochrome c-mediated cell death pathway. This gene and MAPK8 are also known as c-Jun N-terminal kinases. This kinase blocks the ubiquitination of tumor suppressor p53, and thus it increases the stability of p53 in nonstressed cells. Studies of this gene's mouse counterpart suggest a key role in T-cell differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Sep 2008]
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