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CDT2 (DTL) Human Gene Knockout Kit (CRISPR)

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SKU
KN207280RB
DTL - human gene knockout kit via CRISPR, HDR mediated
  $1,657.00
4 Weeks*

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Other products for "CDT2 or NM_001286229"

Specifications
Specifications
Product Data
Format 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control
Vector RFP-BSD
Target Symbol CDT2
Locus ID 51514
Components

KN207280G1, CDT2 gRNA vector 1 in pCas-Guide CRISPR vector

KN207280G2, CDT2 gRNA vector 2 in pCas-Guide CRISPR vector

KN207280RBD, donor DNA containing left and right homologous arms and RFP-BSD functional cassette.

GE100003, scramble sequence in pCas-Guide vector
OTI Disclaimer These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq NM_001286229, NM_001286230, NM_016448
UniProt ID Q9NZJ0
Synonyms CDT2; DCAF2; L2DTL; RAMP
Summary Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1, CDKN1A/p21(CIP1), FBH1, KMT5A and SDE2 (PubMed:16861906, PubMed:16949367, PubMed:16964240, PubMed:17085480, PubMed:18703516, PubMed:18794347, PubMed:18794348, PubMed:19332548, PubMed:20129063, PubMed:23478441, PubMed:23478445, PubMed:23677613, PubMed:27906959). CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication (PubMed:16861906, PubMed:16949367, PubMed:17085480). CDKN1A/p21(CIP1) degradation during S phase or following UV irradiation is essential to control replication licensing (PubMed:18794348, PubMed:19332548). KMT5A degradation is also important for a proper regulation of mechanisms such as TGF-beta signaling, cell cycle progression, DNA repair and cell migration (PubMed:23478445). Most substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, and those containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to their degradation. In undamaged proliferating cells, the DCX(DTL) complex also promotes the 'Lys-164' monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis (PubMed:20129063, PubMed:23478441, PubMed:23478445, PubMed:23677613). The DDB1-CUL4A-DTL E3 ligase complex regulates the circadian clock function by mediating the ubiquitination and degradation of CRY1 (PubMed:26431207).UniProtKB/Swiss-Prot Function
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Other Versions
SKU Description Size Price
GA109846 DTL CRISPRa kit - CRISPR gene activation of human denticleless E3 ubiquitin protein ligase homolog 1 kit
$1,657.00
KN207280 DTL - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN207280BN DTL - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN207280LP DTL - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN407280 DTL - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated. 1 kit
$1,657.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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