CSDE1 Human Gene Knockout Kit (CRISPR)

CAT#: KN207101

CSDE1 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: CSDE1
• Locus ID: 7812
• Components:

  KN207101G1, CSDE1 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: TACAGATCTCTGATAAGTTG

  KN207101G2, CSDE1 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: CTTACAGATCTCTGATAAGT

  KN207101D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  TAAATTTATA TATCTCCTTA TCGGGCATAC GGTAATACGG TAAGAGCAAA TGAGTGAGGA CTTCACTGTT GGCCTCTTTT GGAGTTCTCC ACTCTTTCCT CAGAATACCT GAGTAATACA TGATACTCAT AGACCATCTG ACATACCAAC TCGGTGAAAC CTAGACTATA AATACATAGT AAATATTTAT AAAAGCTCAT TTTTATCTCT GGGCAGAAAA ATAGTCCCAG TTATTTTCTT CTGCACCCCA TTGGGTTATC AGTGTGTACT CTAGGGAGAC TAGTGACTAA AAATGATAGG TTCTATTTAA AGTTGATCGG CATTGAAAAG AAATTGGACT GAATAGTCTC ACTCCAACAA CCATCAAGAG TCTTTGTGGC ATTTTCAGAA TTGTTGATGT CATCGTATGT TTTAATGGCT TTAAAAAAAA TACATTTCAC CTTTTATAGT AATGTTAATT CTATGTATTT CCTGTCAATA GAAATCTAAT TTATATTTCG CTAAACCCAA GCTAAAAATA TATATTGACT GTGAAATGTG CATTAAATTG TTATTGATCA TAGTTATCAA CATTATGCTA TGTTATAACA GCTGTTTGAG CTCAGCTTAC CTTGCTTTGT GTTAAGAGCA TGACCTAGGA TTGCCACTCA TTCTGGTTGA AATATCTTGC CGACTCACCT TCTAACTTCC CGAATCATTT TTTTCACCTT CACTCTTGAA AGATAACTTC AGTAGATTAG GGTCTTACTG CCCAAAGTAT ATCTAAGAAT CAGAAGCATT ATTATCACCT TGGATCTTAA TAAAGATGGA AAATTTCAAA TCCCCATACC TACTGAGTCT GATTTTATAT TTTAACTTCT CATTTGAGAA GCCCTGGTTT ACAGCCAGAC CTTCTTGGTT TGAATCTTCT ACTATTTGAC TTTGGCAAGT CATTTAACTT CTCTGTGTCT GTTTTCTCAT TGGTAAAATG AGGCGAATAC AATACTTTCT TGTGGGCTTT TGTGAAAGTT AAATGAGTTA ATATATGTTA AACACAGAAC AGTGGCTAGT GTAGAAGAAT TAGGACGTAA CCACCTACAG GTGTGGTGGC TTTGATTTAC GTGATTTGAC CATCACACAT ATCGTTGACT CAAACTTTGG TTTATCTTTC TAGAATTTGT TACTCTTTTT TCCTAGTGAA AATATATCCT TTTTATTAAT

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001007553, NM_001130523, NM_001242891, NM_001242892, NM_001242893, NM_007158
• UniProt ID: O75534
• Synonyms: D1S155E; UNR
• Summary: RNA-binding protein. Required for internal initiation of translation of human rhinovirus RNA. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain.[UniProtKB/Swiss-Prot Function]