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CSDE1 Human Gene Knockout Kit (CRISPR)

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SKU
KN207101
CSDE1 - human gene knockout kit via CRISPR, HDR mediated
  $1,657.00
4 Weeks*

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Specifications
Specifications
Product Data
Format 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Vector GFP-puro
Target Symbol CSDE1
Locus ID 7812
Components

KN207101G1, CSDE1 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: TACAGATCTCTGATAAGTTG

KN207101G2, CSDE1 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: CTTACAGATCTCTGATAAGT

KN207101D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

Homologous arm and GFP-puro sequences:

pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

TAAATTTATA TATCTCCTTA TCGGGCATAC GGTAATACGG TAAGAGCAAA TGAGTGAGGA CTTCACTGTT GGCCTCTTTT GGAGTTCTCC ACTCTTTCCT CAGAATACCT GAGTAATACA TGATACTCAT AGACCATCTG ACATACCAAC TCGGTGAAAC CTAGACTATA AATACATAGT AAATATTTAT AAAAGCTCAT TTTTATCTCT GGGCAGAAAA ATAGTCCCAG TTATTTTCTT CTGCACCCCA TTGGGTTATC AGTGTGTACT CTAGGGAGAC TAGTGACTAA AAATGATAGG TTCTATTTAA AGTTGATCGG CATTGAAAAG AAATTGGACT GAATAGTCTC ACTCCAACAA CCATCAAGAG TCTTTGTGGC ATTTTCAGAA TTGTTGATGT CATCGTATGT TTTAATGGCT TTAAAAAAAA TACATTTCAC CTTTTATAGT AATGTTAATT CTATGTATTT CCTGTCAATA GAAATCTAAT TTATATTTCG CTAAACCCAA GCTAAAAATA TATATTGACT GTGAAATGTG CATTAAATTG TTATTGATCA TAGTTATCAA CATTATGCTA TGTTATAACA GCTGTTTGAG CTCAGCTTAC CTTGCTTTGT GTTAAGAGCA TGACCTAGGA TTGCCACTCA TTCTGGTTGA AATATCTTGC CGACTCACCT TCTAACTTCC CGAATCATTT TTTTCACCTT CACTCTTGAA AGATAACTTC AGTAGATTAG GGTCTTACTG CCCAAAGTAT ATCTAAGAAT CAGAAGCATT ATTATCACCT TGGATCTTAA TAAAGATGGA AAATTTCAAA TCCCCATACC TACTGAGTCT GATTTTATAT TTTAACTTCT CATTTGAGAA GCCCTGGTTT ACAGCCAGAC CTTCTTGGTT TGAATCTTCT ACTATTTGAC TTTGGCAAGT CATTTAACTT CTCTGTGTCT GTTTTCTCAT TGGTAAAATG AGGCGAATAC AATACTTTCT TGTGGGCTTT TGTGAAAGTT AAATGAGTTA ATATATGTTA AACACAGAAC AGTGGCTAGT GTAGAAGAAT TAGGACGTAA CCACCTACAG GTGTGGTGGC TTTGATTTAC GTGATTTGAC CATCACACAT ATCGTTGACT CAAACTTTGG TTTATCTTTC TAGAATTTGT TACTCTTTTT TCCTAGTGAA AATATATCCT TTTTATTAAT

GE100003, scramble sequence in pCas-Guide vector
OTI Disclaimer These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq NM_001007553, NM_001130523, NM_001242891, NM_001242892, NM_001242893, NM_007158
UniProt ID O75534
Synonyms D1S155E; UNR
Summary RNA-binding protein. Required for internal initiation of translation of human rhinovirus RNA. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain.UniProtKB/Swiss-Prot Function
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Documents
Resources
Other Versions
SKU Description Size Price
GA105324 CSDE1 CRISPRa kit - CRISPR gene activation of human cold shock domain containing E1 1 kit
$1,657.00
KN207101BN CSDE1 - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN207101LP CSDE1 - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN207101RB CSDE1 - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN407101 CSDE1 - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated. 1 kit
$1,657.00

file-alt Citations

Cold Shock Domain Containing E1 (CSDE1) Protein is Overexpressed and can be Targeted to Inhibit Invasiveness in Pancreatic Cancer Cells
Liu, Li, Dun et al
Proteomics (2020)
Cited by 12
AI Summary: OriGene products, including the CSDE1 human gene knockout kit (CRISPR, KN207101), gRNA vectors (KN207101G1 and KN207101G2), and TurboFectinTM 8.0 transfection reagent, were used to achieve CRISPR-Cas9-mediated knockout of CSDE1 in pancreatic cancer cells, enabling the study of its role in cell invasiveness.
Snippet: CRISPR-Cas9 knockout. CSDE1 human gene knockout kit (CRISPR, KN207101) and transfection reagent TurboFectinTM 8.0 were purchased from Origene (Rockvill, MD). Two gRNA vectors (SKU KN207101G1 and KN207101G2) and the scramble negative control
Showing 1-1 of 1 papers.
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