SLC22A4 Human Gene Knockout Kit (CRISPR)

CAT#: KN206575

SLC22A4 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: SLC22A4
• Locus ID: 6583
• Components:

  KN206575G1, SLC22A4 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: TGGTATGTCAGTCGTGTTCC

  KN206575G2, SLC22A4 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: CGACGAGGTGATCGCCTTCC

  KN206575D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  AGCTCAGCCT CCAGATTGGA GGAGACCGCG GAGGGAACCC TGCTGGGGTC TGGGCCCGGG GCCACGCGGC CCGAGCAGAT CGAGGGCCGA CCCCTCCGAG AACTCGCTCT CTGGCCTCGG CTCCTCCCTT GCGCCCGCCC TCCCACGTGG GGCCCAGGTC TGGGAATCAG CGCTCGGGGG TGGCTGGGGA CAACCGAGAA CGAGCTTCTT CCCCGGCACG CGGGCGGAAT GGCTGAGCCC AGCCTGGAAG CCCCCGTCAG GTCCTTGGGG GCGGGCGGGC GCGCGAAGCA CAGGGCGGAG ACAGCCGGGA GCCCAGCCTC CCGGGCTGGG CCGCCCTCCC CTTCCCCGCG CCCGGCCGGG GATGGGGGTG TGGTCCCAAG TGTACAGTGG CATCAAGCTC AGCGCGAGCT CCCGGGAACG CTCCAACGCC TTCAGCCTGT TTCCCAGGAA CGGTCCCCGG CTTCGCGCCC CAATTTCTAA CAGCCTGCCT GTCCCCCGGG AACGTTCTAA CATCCTTGGG GAGCGCCCCA GCTACAAGAC ACTGTCCTGA GAACGCTGTC ATCACCCGTA GTTGCAAGTT TCGGAGCGGC AGTGGGAAGC ACCGCTGTCG AGTGCCGGAC GCCGCGAACC TGAGCAGCGC CTGGCGCAAC AACAGTGTCC CGCTGCGGCT GCGGGACGGC CGCGAGGTGC CCCACAGCTG CAGCCGCTAC CGGCTCGCCA CCATCGCCAA CTTCTCGGCG CTCGGGCTGG AGCCGGGGCG CGACGTGGAC CTGGGGCAGC TGGAGCAGGA GAGCTGCCTG GATGGCTGGG AGTTCAGCCA GGACGTCTAC CTGTCCACCG TCGTGACCGA GGTGGGTGCC AGGCCGAGAC CGTTGACCCG GGAGTGCCTG ACCCTCCCTC TGCGTCAGCC CCCCTTGAGA CTCCCTGCGC AGTGCCCGGG TCAGCGCTCC CCTCCCCCTC AAACCTGCTG TTCATACTTC CAGCAGCGGG TGTGCACCCC AAGAAAGAAT AGGACTCACG CGAGGCGCAT TCCTGGGTCG TTTCTGTCCA ATAAGGAGGT GATGGAGAAA GGAGTTTGTC ACCAACTGTC TTTTCATTTC CGATTTTCAC AACAAATGAA GTTTCCTAAG AACCTAGATG TTGCGTTGGG GGAAGCGCAG CCCCAGGCTA CCCAGACGCA CTGCCTGAGT CACCTTCCTG

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_003059
• UniProt ID: Q9H015
• Synonyms: OCTN1
• Summary: Polyspecific organic cation transporters in the liver, kidney, intestine, and other organs are critical for elimination of many endogenous small organic cations as well as a wide array of drugs and environmental toxins. The encoded protein is an organic cation transporter and plasma integral membrane protein containing eleven putative transmembrane domains as well as a nucleotide-binding site motif. Transport by this protein is at least partially ATP-dependent. [provided by RefSeq, Jul 2008]