RBM7 Human Gene Knockout Kit (CRISPR)

CAT#: KN206461

RBM7 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: RBM7
• Locus ID: 10179
• Components:

  KN206461G1, RBM7 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: CCTTGAAACGAAAGTGACCG

  KN206461G2, RBM7 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: AAGCTCGAAAAGGAGCTCCT

  KN206461D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  GCTTGGCGGC TCCGGCCCCG GCCATCTGGT TCCCTTGGGC TCCGGCCGCC ACCATCCACT CGACGGCTCT CGGCCCGAAC GCTTGGTCGC ACCGCCTGCC GAGGTCCTAG ATGAATCGCT TCAGGCCTGG AAACGAGGAA GCCGTCTCCG GAGACCATCG CCAACGCTGA CGCCCGCGGT CTGAGGTCGC CATGGGAAGA GCGGTAGGCC ACCCTGCTCC TCTGATCACC GGAGGACAGG GACACATTGT TCAGGGCCAT ATTCAAACAC TGCCCGCAGT ACTTGCGTTA CGTCCCTTTG TGAAGGCAGG CCCTTCGCGG CTCCCCAGAT CAGTCCAGCC TGTGTCGGAC CCGATGACTA AGCACACAGG AACCCATAAC TGAGCCTGCG GAAGAGCCAG AAGCCGCCTT GCCTTTAACG AGGGGTATCC TGGCGAGCAT GCGCAGACCG TCCGCGCACG TCGTCGCCCG CTGAGAGCAA GCGCAACGGG CGTTTTCGTT TGTGACGCCA GGGAGCGTGA GGACGTGGGG CTTCCGTGAA TGCGCAGTGG GTGCGTCGGC CACGACCTTT TGGCCAGGTT AGGGAGGGGG CGACGCTGAG GCTGGGTTCG GCCCTTTGCC TTTCGTTTTC CGTCTCGCCT AGGGCCTGGC CAGCGGCCAC CCCGTTTTCT TTTCGTAGCC GTCAGGGGAC CCGACGGGTG GCTGTTTGGG GGTGAAAGGC GGGTCTGGGT TGCGAAACGC TCGCTGGGTG TCGCTTTCCT GGAAGATCTT GGTTCGTTTA GGCCGAAAGT GACGACTAAA GGTGGTGGAG GGATCCTCGA TCAGGTTTCC CGTGGTAGAG ATCCAGGGGT CCTTGGGAAC ACAGTGTGTG TGTAGGGTCT TCGTTGAGCA GTGCTTTTTC TTATCCTTCC TGTTGTCATC TTCCCCTTAG GATAATTTGT CTTTGGTTTA TCTGACTGGG AACTTAATAT TTTAATTTAT TTGTGTCTGT TTTCCCCTAA AGTTTGCTTA TCAAATTGCA GCATTATTAA AATATGTAAA ATAAAATGGT TAAGTACTCC GTAGAAAAAC AGCATTTTAG TGTTGCTGTA TTAAAAAGTG TGAAAAATTC TGAGTATCCT ATTCCCCCCA TCCCTATCCC CTAATAACTG GTATTGTTTG ATTAATCAAT ACAAAAGTCC GTTTTTAGTT AAAGAAAATC

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001286045, NM_001286046, NM_001286047, NM_001286048, NM_016090
• UniProt ID: Q9Y580
• Summary: Subunit of the trimeric nuclear exosome targeting (NEXT) complex, a complex that directs a subset of non-coding short-lived RNAs for exosomal degradation. The RNA exosome is fundamental for the degradation of RNA in eukaryotic nuclei. Substrate targeting is facilitated by its cofactor MTREX, which links to RNA-binding protein adapters (PubMed:27871484). Possible involved in germ cell RNA processing and meiosis (Probable).[UniProtKB/Swiss-Prot Function]