EIF4A2 Human Gene Knockout Kit (CRISPR)

CAT#: KN205623BN

EIF4A2 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 mBFP-Neo donor, 1 scramble control
• Donor DNA: mBFP-Neo
• Symbol: EIF4A2
• Locus ID: 1974
• Components:

  KN205623G1, EIF4A2 gRNA vector 1 in pCas-Guide CRISPR vector

  KN205623G2, EIF4A2 gRNA vector 2 in pCas-Guide CRISPR vector

  KN205623BND, donor DNA containing left and right homologous arms and mBFP-Neo functional cassette.

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001967
• UniProt ID: Q14240
• Synonyms: BM-010; DDX2B; eIF-4A-II; EIF4A; eIF4A-II; EIF4F
• Summary: ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.[UniProtKB/Swiss-Prot Function]