SHCBP1 Human Gene Knockout Kit (CRISPR)

CAT#: KN205527

SHCBP1 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: SHCBP1
• Locus ID: 79801
• Components:

  KN205527G1, SHCBP1 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GGAAATTTGAAATGGCTGAC

  KN205527G2, SHCBP1 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: ATGGCTGACGGGTCGCTGAC

  KN205527D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  TGTTAGCCAT TATGATGGAG ATTATAGTTG TCTTTGATTA ATGCAAATTA ACAAATTTAC TTACTAAATT TAATTTGACA TTCTTTGAAA ATATGCTCTC TCTCTCTCTC TCTCTCTCTG TGTGTGTGTG TGTGTGTGTG TGTGTATTGA AAGACAGAGA GAGACTGGTG TTTGGTGGTG AAAGTTTTGG GCCCACGTAG GCCAGCATCC TTTTCAGTTG AAGTTGTGCT TGTGGGAGGT GAAATGACTT GCCCAAGGCA ATACCACTAG GAATAGCCCA GGTAAGTTTA AATACTATGT TTTTTGACTC TCAACCCAGT GACTTTTCCA CTATATCTCA TTTCCAGGCA TTGCTGGTTC GTTTTTTTCG AAAATCATTC CAAATTATAT TCCAATTCGA CTTTCTTCAA GTTCATCCCA GAAAAGCAAA GGCCGGGCAC TTGAACAAGA ACAGCTGTCA GCCTGGGGCG AGGGGGCAGT GTTGGTGGGG GCTGGAGCGG GGCCGGACTC TAATTGGTCC ACGGCCTCGG CCCGACTATC AAATCAGCAG AGCCTGCGTC ATCACGTCGT TGCCTGGGCT GCCGTCCGCG GAAATTTGAA GGCGCCGGAG CGCATGGGCT GGGCGGTGGA GCAGGAGCTG GCGTCTCTGG AGAAAGGTGA GCTCTCCATG CCGGGGCCGA GGAGCCGCCT CTGAGCGGAG GCCTGGGGCG TCGGGACCCT AGGAGGGCGC CTGGGTCGCG GGCCTTCCGG ACCCGTCTGT CCAGGGTGGC GGGAACGGCG GTGCGCGGCC GGGACCCAGT GACGGGACCT CAAAGTCGGG CGGTACCCAG GGGTGGGGGA CTCCCTCTCT TCCCTATCCG CATCTCCCAC TCTCCCTGCA CTCTCTGGCC TCCGCCCGCC CCGAGGTTGG AGGGAGCTGG ATTGGGCCAG GAATTCCCTA TCTGGATGGG TTTTGCGGGA ACTGTGCATT TACTGTTGCT CAAGGGGCCA TTTTTTCATC TGCATCTCAA AGGAGATCAC GAACCACACT TCCCTTTTCC GAAGAAGAGA AAAGCTTGAA CCCTTGGCTT AGATGGCTGT CCTGTGCACG GAGGGTAGTG ACGTGTCCAA AGTGAGGAGC GCCAACTTGG GCCGGAGTCC AGAAATAGAA CCGGGGTCTT CTAGTTCTCT GCCCTTTCTT CCTCCTCATA GAGTTTCATT

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001324318, NM_001324319, NM_024745, NR_136738
• UniProt ID: Q8NEM2
• Synonyms: PAL
• Summary: May play a role in signaling pathways governing cellular proliferation, cell growth and differentiation. May be a component of a novel signaling pathway downstream of Shc. Acts as a positive regulator of FGF signaling in neural progenitor cells.[UniProtKB/Swiss-Prot Function]