ISLR Human Gene Knockout Kit (CRISPR)

CAT#: KN205281

ISLR - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: ISLR
• Locus ID: 3671
• Components:

  KN205281G1, ISLR gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: TACTTTTCCCCACAGTCGCA

  KN205281G2, ISLR gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GCTCTGGTGGGCGCTTCTCC

  KN205281D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  GGGGGTTTAT TTAGGCAGCT GCCTGGCTAA GTTTGAACAG AACAGGCCAC GGGTGTGATT CCACAGAAAA GGCCTGGTGT CTGCTGCGGT CATGGCCGGA GGAGCGGGAG AGGGCGGGTG GAGTGGATGG GGGTGGTGTG CACTGCACAA GGGGCCTCGT CTGGGCCAAG GCAAAGCATA CCTATGGGGG GCTCCGGTGG GAGGGACTGC GGCCAGGATG TGGGAGGGCA GGGGGAGGTT CTGCAAAGTG CTGGGGGAGG GGGGTGGCTG GAAAACAGAT TTCAAGTCAT AAAGTCAGCT AGGAACAGGC CGAGGCAGGG AGAACTCTCC ACTCGGAGGA GGAGCTGGGG TCCTCTTCCA TCCCGTCTTC ATCCTGCCTG GCTGCGTGAC CTCGGGCAAG TCTCCGCCCT TCTCTTGGCC TCAGTTTCTC CTTCCGTAGG ATGGGGGCGG TGGGCTAGGT GGTGTTGGGA TTTAGCTGGG TTATGTGGGA CAGGGCCTCC TGATGGGAAA GAGCTCTGGC TGGGCTTGTG GGAGGAATGA GTCCCTTTGG CAGGTTCTCG GGATCCCCTG GGTGACATGC CTTTCTCTGC AGGAGGCACC TCCGTGCCGC CTGGCTTCCC GGCCAATGTG ACTACACTGA GCCTGTCAGC CAACCGGCTG CCAGGCTTGC CGGAGGGTGC CTTCAGGGAG GTGCCCCTGC TGCAGTCGCT GTGGCTGGCA CACAATGAGA TCCGCACGGT GGCCGCCGGA GCCCTGGCCT CTCTGAGCCA TCTCAAGAGC CTGGACCTCA GCCACAATCT CATCTCTGAC TTTGCCTGGA GCGACCTGCA CAACCTCAGT GCCCTCCAAT TGCTCAAGAT GGACAGCAAC GAGCTGACCT TCATCCCCCG CGACGCCTTC CGCAGCCTCC GTGCTCTGCG CTCGCTGCAA CTCAACCACA ACCGCTTGCA CACATTGGCC GAGGGCACCT TCACCCCGCT CACCGCGCTG TCCCACCTGC AGATCAACGA GAACCCCTTC GACTGCACCT GCGGCATCGT GTGGCTCAAG ACATGGGCCC TGACCACGGC CGTGTCCATC CCGGAGCAGG ACAACATCGC CTGCACCTCA CCCCATGTGC TCAAGGGTAC GCCGCTGAGC CGCCTGCCGC CACTGCCATG CTCGGCGCCC TCAGTGCAGC TCAGCTACCA ACCCAGCCAG

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_005545, NM_201526
• UniProt ID: O14498
• Synonyms: HsT17563