Fas Ligand (FASLG) Human Gene Knockout Kit (CRISPR)

CAT#: KN204894

FASLG - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: Fas Ligand
• Locus ID: 356
• Components:

  KN204894G1, Fas Ligand gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: ATATCCCCAGATCTACTGGG

  KN204894G2, Fas Ligand gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: CTGGGCACAGAGGTTGGACA

  KN204894D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  TTTATAGTTT AAAAAATACG GTTCTGAACA ATTTTTGTTA GAGTTATTTT GGGATTTTTA AATAGTTTTT TGGTTGTGTA TATGGTAGAA TTCTTAAAAT TATACAATTA TAATGTATAA AAAAGCATGC AATTATAATT CATAAAATTA TAGCCCCACT GACCATTCTC CTGTAGCTGG GAGCAGTTCA CACTAACAGG GCTATACCCC CATGCTGACC TGCTCTGCAG GATCCCAGGA AGGTGAGCAT AGCCTACTAA CCTGTTTGGG TAGCACAGCG ACAGCAACTG AGGCCTTGAA GGCTGTTATC AGAAAATTGT GGGCGGAAAC TTCCAGGGGT TTGCTCTGAG CTTCTTGAGG CTTCTCAGCT TCAGCTGCAA AGTGAGTGGG TGTTTCTTTG AGAAGCAGAA TCAGAGAGAG AGAGATAGAG AAAGAGAAAG ACAGAGGTGT TTCCCTTAGC TATGGAAACT CTATAAGAGA GATCCAGCTT GCCTCCTCTT GAGCAGTCAG CAACAGGGTC CCGTCCTTGA CACCTCAGCC TCTACAGGAC TGAGAAGAAG TAAAACCGTT TGCTGGGGCT GGCCTGACTC ACCAGCTGCC GAGGCCACCA CCACCACCGC CACCGCCACC ACTACCACCT CCGCCGCCGC CGCCACCACT GCCTCCACTA CCGCTGCCAC CCCTGAAGAA GAGAGGGAAC CACAGCACAG GCCTGTGTCT CCTTGTGATG TTTTTCATGG TTCTGGTTGC CTTGGTAGGA TTGGGCCTGG GGATGTTTCA GCTCTTCCAC CTACAGAAGG AGCTGGCAGA ACTCCGAGAG GTAAGCCTGC CGGCAGACTG CTGTGCCCTG GAGGCACCAG GCATAAGGGG ATGGAGGGCC CACTGCCTGG CTTGCAAAGT GCTTTTCAAT CCTTTTTTTT TTTTTCTTAG AAATGGGTTG TTCTAGTTAT TCTATTCTAT AGACAGAGAA ATGGAGGCTC ATAGGGGTGA AGGGAATATT TTTTACTTTG TAAAGAATCA GAGCCCAACA GTTTGTCTTT CCTCAGTTGT CAACTCAGTC TGTTGAACCA CTTACTGCCT CATACCTATT GATTTAAGGA GTAAAAAACA AACAAACAAA ACTTTTAAGT TTTGCTTGGA AGTAAATTTG TTCAGATGAC CAAACTAAAT CTTGAAAATT GGTACGATTC CAAATCACAA

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_000639, NM_001302746
• UniProt ID: P48023
• Synonyms: ALPS1B; APT1LG1; APTL; CD95-L; CD95L; CD178; FASL; TNFSF6
• Summary: This gene is a member of the tumor necrosis factor superfamily. The primary function of the encoded transmembrane protein is the induction of apoptosis triggered by binding to FAS. The FAS/FASLG signaling pathway is essential for immune system regulation, including activation-induced cell death (AICD) of T cells and cytotoxic T lymphocyte induced cell death. It has also been implicated in the progression of several cancers. Defects in this gene may be related to some cases of systemic lupus erythematosus (SLE). Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2014]