ThPok (ZBTB7B) Human Gene Knockout Kit (CRISPR)

CAT#: KN204105

ZBTB7B - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: ThPok
• Locus ID: 51043
• Components:

  KN204105G1, ThPok gRNA vector 1 in pCas-Guide CRISPR vector

  KN204105G2, ThPok gRNA vector 2 in pCas-Guide CRISPR vector

  KN204105D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001252406, NM_001256455, NM_015872, NR_045515, NR_046206, NR_049765
• UniProt ID: O15156
• Synonyms: c-KROX; CKROX; hcKROX; THPOK; ZBTB15; ZFP-67; ZFP67; ZNF857B
• Summary: This gene encodes a zinc finger-containing transcription factor that acts as a key regulator of lineage commitment of immature T-cell precursors. It is necessary and sufficient for commitment of CD4 lineage, while its absence causes CD8 commitment. It also functions as a transcriptional repressor of type I collagen genes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]