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c20orf72 (MGME1) Human Gene Knockout Kit (CRISPR)

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SKU
KN203886
MGME1 - human gene knockout kit via CRISPR, HDR mediated
  $1,657.00
4 Weeks*

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Specifications
Specifications
Product Data
Format 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Vector GFP-puro
Target Symbol c20orf72
Locus ID 92667
Components

KN203886G1, c20orf72 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: AGACCATTTGCAGGCAGCTC

KN203886G2, c20orf72 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: TGTGGAATCAGCTGCCCTTG

KN203886D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

Homologous arm and GFP-puro sequences:

pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

CGGGAGGCGA CTTTTTGCGG TCACAGTAAT GGGACCTCAG CGCCGCCACC AGCTCTGACC GGCGAGCGAC GGGACCCTCC AACCCGATTT CCACGTCTGT GCAACGGGTT CATTCTAGAC CCACGGTAGA GGGTGTGAGG ACCAAATGAG AAGTATCCAC ACAGAATACT TAGCAGAGAG CCTGGTGTCT AGGAATTAGT CGTCGTCTGT CACCTAGGAC TGTGTGGCCG CCATGGGACG GGCTCCATCT TTGTGTTTTT CCGTGTCTCG CACACGCCTC CCTTCCTGGG CGTCACATTC CTGCAGATTA GAAACTGGGA CGTCTTGCCT CAAGCTGTCT ACTAATTCGC ATTTAGTATG TGTCGGTCCT TCGGGTATTG AGTCTTGATT TATTTATAAA TTTTTTTTGG AGGCAGGGTC TCGCTCTGTC GACTTTCTTA AGAAAGGGCT CTGTCCAAAC ATGTCGAAAA AGTTCACAAT TTTGATGTGC AATATCATTC TGATGCAGCT TTCGCTTTGA TGGTTGTTTT CTCTCCAATA GGAATACAAA CATAAAGGCC TTCGACCGTT GCAAATAGAC TAAAGTGAAA ACAAATCTGA TGGCTTTCTC TACTTCCTCT TACTCATGTG GCCGGAAGAA AAAAGTGAAC CCATATGAAG AAGTGGACCA AGAAAAATAC TCTAATTTAG TTCAGTCTGT CTTGTCATCC AGAGGCGTCG CCCAGACCCC GGGATCGGTG GAGGAAGATG CTTTGCTCTG TGGACCCGTG AGCAAGCATA AGCTGCCAAA CCAAGGTGAG GACAGACGAG TGCCACAAAA CTGGTTTCCT ATCTTCAATC CAGAGAGAAG TGATAAACCA AATGCAAGTG ATCCTTCAGT TCCTTTGAAA ATCCCCTTGC AAAGGAATGT GATACCAAGT GTGACCCGAG TCCTTCAGCA GACCATGACA AAACAACAGG TTTTCTTGTT GGAGAGGTGG AAACAGCGGA TGATTCTGGA ACTGGGAGAA GATGGCTTTA AAGAATACAC TTCAAGTAAT TATCTCAATT CTGATTCTAT ATGTTTGCTA TGTTTTCTAT AATAGAGAGC AACGGTGTCA AAAGAATGAG GTTTGGTTTT GTTTTTTTAA CTTACTAGCA AGGAACTCCC TTCAGATAGT AATTAACATT AAATAGCACT TTATGTGTAT TAACTCACTT

GE100003, scramble sequence in pCas-Guide vector
OTI Disclaimer These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq NM_001310338, NM_001310339, NM_052865, NM_001363738
UniProt ID Q9BQP7
Synonyms bA504H3.4; C20orf72; DDK1; MTDPS11
Summary The protein encoded by this gene is a nuclear-encoded mitochondrial protein necessary for the maintenance of mitochondrial genome synthesis. The encoded protein is a RecB-type exonuclease and primarily cleaves single-stranded DNA. Defects in this gene have been associated with mitochondrial DNA depletion syndrome-11. Three transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jul 2015
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Documents
Resources
Other Versions
SKU Description Size Price
GA114229 MGME1 CRISPRa kit - CRISPR gene activation of human mitochondrial genome maintenance exonuclease 1 1 kit
$1,657.00
KN203886BN MGME1 - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN203886LP MGME1 - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN203886RB MGME1 - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN403886 MGME1 - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated. 1 kit
$1,657.00

file-alt Citations

Replication fork rescue in mammalian mitochondria
Torregrosa-Muñumer, Hangas, Goffart et al
Sci Rep (2019) 9 (1), 8785 DOI: 10.1038/s41598-019-45244-6
AI Summary: OriGene contributed to the study by providing the plasmid vector carrying the MGME1-targeting sgRNA and Cas9 (CRISPR/Cas9 plasmid), which was used to generate MGME1 knockout HEK293 cells for investigating mitochondrial DNA replication fork rescue mechanisms.
Snippet: MGME1 knockout (KO) HEK293 cells were generated using the Crispr-Cas9 approach, as described previously37. The plasmid vector carrying a single guide RNA (sgRNA) sequence for MGME1 (5′-AGACCATTTGCAGGCAGCTC-3′) and coding for Cas9 were purchased from Origene
FigureFigureFigure
Showing 1-1 of 1 papers.
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