TOE1 Human Gene Knockout Kit (CRISPR)

CAT#: KN203377RB

TOE1 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control
• Donor DNA: RFP-BSD
• Symbol: TOE1
• Locus ID: 114034
• Components:

  KN203377G1, TOE1 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: AACTGCGCCATCGTCACTGT

  KN203377G2, TOE1 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GACGGTGAGCGGCTTCCCAG

  KN203377RBD, donor DNA containing left and right homologous arms and RFP-BSD functional cassette.

  Homologous arm and RFP-BSD sequences:

  pUC vector backbone in gray; Left arm sequence in blue; RFP-BSD in green; Right arm in violet

  CTTCCCTACC AATCGGTCGC TCTTACACCC TGGGCAGCCT CCTCCGATGG CCCACTCCCC GCCTCTTTCA CTTTCTGGAG ATCACTGAGC TCTCCATCCT CTCTGGGAAT TTACCGATGC CCAGAACGCC CTTCTTTCCC CCACACGACC CTCTCCTAGT CTAACTCCTG GGCGTGCTTT AAGCTCAGCT CAGGCAGCGT CACCTTCTCT GGAAAGCCCA AACCCAGCCA CCCCACTACC CGCTACCCGC GGCCCACGCT GATGAAGACA GCAGAACACG GAGGCCCCGC GTTCCCGCCG CGAGAGCAGG AGAGAAAGAT TACCTCCCGC GAGCTCTAGC GCGCCCGGCT TTCCGGCGCA CTCCAGGGGG CGTGGCTCGG GTCCACCCGG GCTGCGAGCC GGCAGCACAG GCCAATAGGC AATTAGCGCG CGCCAGGCTG CCTTCCCCGC GCCGGACCCG GGACGTCTGA ACGGAAGTTC GACCCATCGG CGACCCGACG GCGAGACCCC GCCCCATCCC CGACTGCCTG AACCGCGCCA GGAGACGGAC CGCAAGTCCA GCGTACCCAC AGACGACTCA GGCGGGAGAC GAGCGGTGTC CCTCTGCGCT CTGGGAGAGG GGAAGGCCTC GGGCTCATAG TTCTAGAGGC TCCTCAAGCT TCAGAGGACT GCTCCTTCCG CCTGAACTAG CCACGAGGAG ACTACAAGTT CCGTTGTACA CCGCGGCTCC GGCTGCAAAA GCCTGGAGCG TTGGGAGCAT GGGGCTGATG GAGGCATGGC GGGAGATGTA GTCTGGAGCT CATAACGGTT AGTAGTAATA GTTGTGATTC AGTTCAGTGG AGTGGATTGA GCGTTGTGAT CCAAAGGAAG GTAGGACGTA TTGGGAGCTC TGGTGTGGGG CACTGCCAGG CTCTGGGGCT TACTGGGAGC TGTTGCTTGT GGCGCTGGGG CACCATGAGA ACAGTAGTAT GGATCCCTGT GGGATATTAA GGGAGCTGAG ATCCTCAAGC CCTACAAAAT GTTTCCTGCA GGACATGGGA AAAAAGAATC AAAGGTCGTC TAGTCCAACT TTCTCCTCTA CAGATTGTGA AAGCGAGGTG GGAAGTAATT TACCCAGGCT CATACAAGCC AGTAGAAGAC TCAGGCTTCC TCTCCCCAAA TTCAGGGCAT TCTCCACTCC AAGGCCTGTG TGTAGAACAC

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_025077
• UniProt ID: Q96GM8
• Synonyms: FLJ13949
• Summary: Inhibits cell growth rate and cell cycle. Induces CDKN1A expression as well as TGF-beta expression. Mediates the inhibitory growth effect of EGR1. Involved in the maturation of snRNAs and snRNA 3'-tail processing (PubMed:28092684).[UniProtKB/Swiss-Prot Function]