ERCC8 Human Gene Knockout Kit (CRISPR)

CAT#: KN203124

ERCC8 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: ERCC8
• Locus ID: 1161
• Components:

  KN203124G1, ERCC8 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GTCCGCACGCCAAACGGGTT

  KN203124G2, ERCC8 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GGAGAGCAGAGTCAACACGG

  KN203124D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  GGACCGCGGA CGCACGAGGC CGGGCCAAGG CGCCCAGAGC CGGGCCGGGT AGGGCTCCGA TGCCAGAGTG AAGGGGTCGA CAGGGGAGAA CGAACTCGGG AAATTCTCTC CGGTGACACC CTAAGTGTCG CATGATGCGT TAGTTGACTA GGGGCTCTCA CTTTTCCCTC CTTTACTGAC CTCGCAATCA CTGACCACGC AATCGCTGCC CACGCCGCCA CCTCACCTCT CCAGTTCTTG TACTGCGGGA TGTAGTAGTA TTTGTTCCCG AATTGGTCCG TGCCCACGTG CTCCTTCACT TCCCTTGACA GCGATCTCCA CAAGGCGCGG AACAAATCCT GAGACCAACC CATGCCGTCC ACCCAGTTTC CAGCGCTGCC AGCAGCGGGA CCCGCAGTAG GGGTAATGCT CCAGCCGCCG ACCCAGCCCA CCGAGGCGGA GAAGTCAGGT CGGCCTCCAT CCTGGAGCCC TTCTAGTAAC GGCGCTCACC ATTGGCCGGA TATATCACCG ACTGACCAAT GGGAGACTCT GCTGTTCCAG TCCCGCCCCT GAGCTCTGTC ATGGCGACGT CCAGTGCTCC AGCCGGTGTG AGGACACGAT AGGTAAAGAA AACCTTACTT TTTGGCTAAC AGGCCAGTTA GAATCTGGAC CAATGACTGT AAGCTTTGCC CCACACTTTC CCGTCTCATC GCTCTCCTGC CACCAAACTA TTATTTGCCC TCTAATGCTG TGTGGCTTGC TAAAATAGGA TCCCTTTTAT TTACTGGTAA CAACTTGCAG GCGGGGGGCG GCGGGGGTGG GGGGGGTTCC CCGGGGGTGG GGGGGGGGTT CCCCCGCGGG CTCAGGTGAA GACACTATAA ACCTAAATAA GAATACTTCG TTGTACTGTA TTCCTTCTCT CTTAAGTTAC TCCAAAGAAT AACTGCCCTC CACGGACTCG TTCATTCATT CAGTCTGCAT ATTTAGTGAA TTCCTACAGT GTGCCAGGCA GCCTATTAAA GGCAGAGGAC ACAGGCCAGG AATTGAACAA AGTTCAGAAT GACTAGAGCG GAGAATTGGG GTAGGGACCA TTGACAAGAG ATGAAGTAGA CAGAAACTAG ATGAGGAAGA GCCTTGTGTA CTTGAGTAGA GGTGTGAAGA TGAGAATAAG GTAACAGCTA TTGCATTTTG TTTTCTGCTT GGAGAATGGG TTGCAACTGA

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_000082, NM_001007233, NM_001007234, NM_001290285
• UniProt ID: Q13216
• Synonyms: CKN1; CSA; UVSS2
• Summary: This gene encodes a WD repeat protein, which interacts with Cockayne syndrome type B (CSB) protein and with p44 protein, a subunit of the RNA polymerase II transcription factor IIH. Mutations in this gene have been identified in patients with hereditary disease Cockayne syndrome (CS). CS cells are abnormally sensitive to ultraviolet radiation and are defective in the repair of transcriptionally active genes. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2014]