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PTEN Human Gene Knockout Kit (CRISPR)

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SKU
KN202627RB
PTEN - human gene knockout kit via CRISPR, HDR mediated
  $1,657.00
4 Weeks*

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Other products for "PTEN or NM_000314"

Specifications
Specifications
Product Data
Format 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control
Vector RFP-BSD
Target Symbol PTEN
Locus ID 5728
Components

KN202627G1, PTEN gRNA vector 1 in pCas-Guide CRISPR vector

KN202627G2, PTEN gRNA vector 2 in pCas-Guide CRISPR vector

KN202627RBD, donor DNA containing left and right homologous arms and RFP-BSD functional cassette.

GE100003, scramble sequence in pCas-Guide vector
OTI Disclaimer These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq NM_000314, NM_001304717, NM_001304718
UniProt ID P60484
Synonyms 10q23del; BZS; CWS1; DEC; GLM2; MHAM; MMAC1; PTEN1; TEP1
Summary This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms. provided by RefSeq, Feb 2015
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Documents
Resources
Other Versions
SKU Description Size Price
GA103894 PTEN CRISPRa kit - CRISPR gene activation of human phosphatase and tensin homolog 1 kit
$1,657.00
KN202627 PTEN - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN202627BN PTEN - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN202627LP PTEN - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN402627 PTEN - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated. 1 kit
$1,657.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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