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HIF-1 alpha (HIF1A) Human Gene Knockout Kit (CRISPR)

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SKU
KN202461LP
HIF1A - human gene knockout kit via CRISPR, HDR mediated
  $1,657.00
4 Weeks*

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Specifications
Specifications
Product Data
Format 2 gRNA vectors, 1 Luciferase-Puro donor, 1 scramble control
Vector Luciferase-Puro
Target Symbol HIF-1 alpha
Locus ID 3091
Components

KN202461G1, HIF-1 alpha gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: TTTCTTGTCGTTCGCGCCGC

KN202461G2, HIF-1 alpha gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: AGGCGGCGGGCCGAGGGAAT

KN202461LPD, donor DNA containing left and right homologous arms and Luciferase-Puro functional cassette.

Homologous arm and Luciferase-Puro sequences:

pUC vector backbone in gray; Left arm sequence in blue; Luciferase-Puro in green; Right arm in violet

ATCTCCTTTC TCTTTCCTCC GCCGCTAAAC ACAGACGAGC ACGTGAGCGT CGCAGCCCGT CCCAGCTGTG CCTCAGCTGA CCGCCTCCTG ATTGGCTGAG AGCGGCGTGG GCTGGGGTGG GGACTTGCCG CCTGCGTCGC TCGCCATTGG ATCTCGAGGA ACCCGCCTCC ACCTCAGGTG AGGCGGGCTT GCGGGAGCGC GCGCCGGCCT GGGCAGGCGA GCGGGCGCGC TCCCGCCCCC TCTCCCCTCC CCGCGCGCCC GAGCGCGCCT CCGCCCTTGC CCGCCCCCTG ACGCTGCCTC AGCTCCTCAG TGCACAGTGC TGCCTCGTCT GAGGGGACAG GAGGATCACC CTCTTCGTCG CTTCGGCCAG TGTGTCGGGC TGGGCCCTGA CAAGCCACCT GAGGAGAGGC TCGGAGCCGG GCCCGGACCC CGGCGATTGC CGCCCGCTTC TCTCTAGTCT CACGAGGGGT TTCCCGCCTC GCACCCCCAC CTCTGGACTT GCCTTTCCTT CTCTTCTCCG CGTGTGGAGG GAGCCAGCGC TTAGGCCGGA GCGAGCCTGG GGGCCGCCCG CCGTGAAGAC ATCGCGGGGA CCGATTCACC TCGGCGTTAA TGGGATTGGG GGGGGCAGCC TTTTTGTTTC TGCTGCTGCT CCCCTCCCCT CTCTTCCCCC AACCTCGCCG GCCGGGCTCC CCCGCTGTCC ACGTCGCCAT CTTGTCGTGG GGGGTGGGAG ACGCCTCGAA AGTGCTTTCA GGGGCCGGGG TCTGAGCCCT GCTTGCCCTC CCCGCCGGCC GTGGGGGCCT CGCGCCGCCC ACCTACCCGC CTCAAAAACC CAGCCTGCTC TGTGGCCCCA TCCGGAGGGG ACTTTACCCA GCCTGAAAAC CCCGGGAAGA GAAATGAGCT GCAGCTCGGT AGCCGCGGTT TGCACCCGGA GCTTCCGCTC CTTCCCGCCC CCATCCTCTC CAGTTCCATT GAAAACTCGG CCCTGGGGCG GACCCTGCAC GCTGGTCCTG GCTTTCCAGT GGACTTGGGG CCTTGAGTTC CCGACTGAGG GACTCGCGTG GTCGGATGCG ATCTTGTCCT GTAGTTGTCC AGCCGTCGCG GGTGTCTTTG CCTTTGTGCA TTAGGGATTT GCCGCGATGG CCTTAAGATG CGAACTTTTT AGTTTGCACG TGCAGGTTTT GTTTCGTTTT AATCGCCTTG

GE100003, scramble sequence in pCas-Guide vector
OTI Disclaimer These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq NM_001243084, NM_001530, NM_181054
UniProt ID Q16665
Synonyms bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; HIF1; HIF1-ALPHA; MOP1; PASD8
Summary This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. provided by RefSeq, Jul 2011
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Documents
Resources
Other Versions
SKU Description Size Price
GA102107 HIF1A CRISPRa kit - CRISPR gene activation of human hypoxia inducible factor 1 subunit alpha 1 kit
$1,657.00
KN202461 HIF1A - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN202461BN HIF1A - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN202461RB HIF1A - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN402461 HIF1A - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated. 1 kit
$1,657.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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