SUV39H1 Human Gene Knockout Kit (CRISPR)

CAT#: KN202428

SUV39H1 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: SUV39H1
• Locus ID: 6839
• Components:

  KN202428G1, SUV39H1 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GCTGCAGGACCTGTGCCGCC

  KN202428G2, SUV39H1 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GGTTCCTCTTAGAGATACCG

  KN202428D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  GCTTGATGTC CCCTGGCCAC GCTGGGGGTG TTAGGTGATG TATGGGATAT CCTAGAACTA GTCTTGTAAA ATCCAAAAAA TTTTGAATTC CAAAACACAT CTGGCCCAGG AGTTTGAGAT AAGGGATTAA AGACCAGTCT TTCCAGTTAC GCCTTTGTTT GGTGCCCTGG AGGTTTTAAT CTTGTCCTCC CATCCGCCCC ACCTCGGGGC ACTGCATCCT ACTGAGATGG GGGGTTGTTC TCTTTCTTTT GCAAAGTGGA GAGAGGGGTG ATCAGGTCCT GAACCCAAAG CAGGTTTGCA GGCAGTTTTA GGAATGACAT GGAGTTTGAA GCTCTGGAAA TAGATTCCTT TAGGACAATT CATGTTGCAA ACCCCGCTTA GAGGTCTGCT GCTCCAGGGG CAGCTTTGGG CAGTGAGTAT TGGGGTTCAA AGCACATTTC TGTTGTAGAA CAGTCTCAGC ACTGGGGAAG GCCTGTGGAT TAAATTTCCC TGACTCTTAG TCAGTCTTTG GGATGTCCCA GGGAGGATTT GGGGTCCCCT TTGACTCCTG GCCTAGTCAG GCTGCCCAGT AATAGTTCTT TCTGCCCCGC TTGATCCAGG TCGAGTACCT GTGCGATTAC AAGAAGATCC GCGTGAGTCT GGGGTGACCA TGCTCTGGGA GGGGACAGGT CACAAGGAAG TTAGTGTCCT GCCCTCTTGT CCCCAACTGC CCACATCTGT TTCCAAAGGG AATTTTCTCC TTTGACCCTG AAAACACAGG ATGAATAGAC CCTTTTATCC CTATGTTACA GATAGGGAAA CTAAGTAGCT TTCTGGGCAG GAGCCGGAAT GAATTCCCAA ATAAATCTCT TCCCATCTCC CTAGGAATAC AAAGTAATTG CAGAGATTGA GATGCTGAAC CCTGGAGTGG AAATGTCAAT AGCTAATGGC TATTGAGCAC TTTCAGTGTG CCAGGCGTTT CCTCTCGGAA ACTCATTTAA TCCCCTAACA ACTGAAATGG GGATAGTTAT TGCCAGAAGG GGGAGTGGCA GGATGGTTAG GAGAACAAAC TGGAGTCAGC ATGCTTGGGT TCCAGTCCTG GTTGTGTTAC CCCAGGCTAC CTACCCAAAC CTTCAGTACT TCATTTTCCT TGTCGTAAAA GAGGGATAAC TAGGAGCATC TACCTCATGT GGGGTTGTGA GGATTAAGTT AGTTAATAAT

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001282166, NM_003173
• UniProt ID: O43463
• Synonyms: H3-K9-HMTase 1; KMT1A; MG44; SUV39H
• Summary: This gene encodes an evolutionarily-conserved protein containing an N-terminal chromodomain and a C-terminal SET domain. The encoded protein is a histone methyltransferase that trimethylates lysine 9 of histone H3, which results in transcriptional gene silencing. Loss of function of this gene disrupts heterochromatin formation and may cause chromosome instability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]