TRIM29 Human Gene Knockout Kit (CRISPR)

CAT#: KN201916BN

TRIM29 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 mBFP-Neo donor, 1 scramble control
• Donor DNA: mBFP-Neo
• Symbol: TRIM29
• Locus ID: 23650
• Components:

  KN201916G1, TRIM29 gRNA vector 1 in pCas-Guide CRISPR vector

  KN201916G2, TRIM29 gRNA vector 2 in pCas-Guide CRISPR vector

  KN201916BND, donor DNA containing left and right homologous arms and mBFP-Neo functional cassette.

  Homologous arm and mBFP-Neo sequences:

  pUC vector backbone in gray; Left arm sequence in blue; mBFP-Neo in green; Right arm in violet

  CCCATCCTTC CCCCTTAAGG TGAAAAGCAG CGATACAGTC CCAGGGTGGA CAATCTTTCC TCTGAAACTC AGGCCTGCAA ATTACAAGGC TATTTGGCTT GTGTGACCCA GTTAACAGCC CTGTGTCTGG TAGGCCTCTG CAGACACCTC ACCTTCTGCC CCAGGGGCCT CTGGGCTCAG TGAAGCCTGC CCACTTGTGA AGACGGAGAC TCCCCGCTCC CCGGATGAGA AGCAAGTGCG TCAGAGGGAA GCAGCCCAAC TGGTTGCTGA GTTGTGGAAA ATAAGCTGGG GGAGAAAAAA CACTTCTTTT AAAAATCAGG TTAGGGATGA ATGTGCAGGG CGTGGTTCCT GTGCAATTAT CTGCTCTGCT GGCTGCGGGG CTCCCTGCTT CTCCCGGGCC GCTGTGTCAG GCTTGTGCCT GTTGCTCCTC CCCTCCACTC CTCCCCCTCC TCCTCCTCCG CCTCCTCCCC CTCCTCCTCC TCACAGGTGT GTCTCTAGTC CTCGTGGTTG CCTGCCCCAC TCCCTGCCGA GACGCCTGCC AGAAAGGTCA CCTATCCTGA ACCCCAGCAA GCCTGAAACA GCTCAGCCAA GCACCCTGCG GCCTGGAGAA TGGCACCAAG GCTGACGGCA AGGATGCCAA GACCACCAAC GGGCACGGCG GGGAGGCAGC TGAGGGCAAG AGCCTGGGCA GCGCCCTGAA GCCAGGGGAA GGTAGGAGCG CCCTGTTCGC GGGCAATGAG TGGCGGCGAC CCATCATCCA GTTTGTCGAG TCCGGGGACG ACAAGAACTC CAACTACTTC AGCATGGACT CTATGGAAGG CAAGAGGTCG CCGTACGCAG GGCTCCAGCT GGGGGCTGCC AAGAAGCCAC CCGTTACCTT TGCCGAAAAG GGCGAGCTGC GCAAGTCCAT TTTCTCGGAG TCCCGGAAGC CCACGGTGTC CATCATGGAG CCCGGGGAGA CCCGGCGGAA CAGCTACCCC CGGGCCGACA CGGGCCTTTT TTCACGGTCC AAGTCCGGCT CCGAGGAGGT GCTGTGCGAC TCCTGCATCG GCAACAAGCA GAAGGCGGTC AAGTCCTGCC TGGTGTGCCA GGCCTCCTTC TGCGAGCTGC ATCTCAAGCC CCACCTGGAG GGCGCCGCCT TCCGAGACCA CCAGCTGCTC GAGCCCATCC GGGACTTTGA GGCCCGCAAG TGTCCCGTGC

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001330382, NM_012101, NM_058193
• UniProt ID: Q14134
• Synonyms: ATDC
• Summary: The protein encoded by this gene belongs to the TRIM protein family. It has multiple zinc finger motifs and a leucine zipper motif. It has been proposed to form homo- or heterodimers which are involved in nucleic acid binding. Thus, it may act as a transcriptional regulatory factor involved in carcinogenesis and/or differentiation. It may also function in the suppression of radiosensitivity since it is associated with ataxia telangiectasia phenotype. [provided by RefSeq, Jul 2008]