TWEAKR (TNFRSF12A) Human Gene Knockout Kit (CRISPR)

CAT#: KN201041

TNFRSF12A - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: TWEAKR
• Locus ID: 51330
• Components:

  KN201041G1, TWEAKR gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GGAGCAAGCGCCAGGTACGC

  KN201041G2, TWEAKR gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GCTCGCTGCGCCGGTTGCTG

  KN201041D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  TCACTCTGTC GCCCAGGCTG GAGTGTAGTG GCGCGATCTC GGCTCACTGC AACTTCTGCC TCCCGGGTTC AAGAGATTCT CCTGCCTCAG CCTCCTGAGT AGCTCGGATT GCAGGCGCGC GCCACCACGC CCGGCTAATT TTTGTATTTC GAGTAGAGAC GGCGTTTCAC CACGTTGGTC AGGCTGCTCT CGAACTCCTG ACCTCGTAAT CCGCCTGCCT CAGACTCCCA AACTGATGGG ATTAAAGGCG TGAGCCACCG CGTCCGGCCG TTCGTGTCTG ATTTCTATAT GTGCTGCCGA AGCGAGCACT CGTGTCTAAT TTTTGATTTC CCCACACCAC GCACGCTGGA CTAGATCACC GGCTGGGCAA GGAAGGGGGT CTGCGTCCCT GCGGGGTCCT GGCAGCTCCC GCGCCAGGAC TTTGTTGAAT GAATGACTGA ACTGAGCGGG CGCCGCGGGG CGGGGCGTCC CGAAGCGGAC CTCAAGGCGG GCGGAGGCGA GAGCTCCGCC CCGGAAGGCG GGGGCGGGGG CGGGGCGGCG GCCGTGGGTC CCTGCCGGCC GGCGGCGGGC GCAGACAGCG GCGGGCGCAG GACGTGCACT GGGACTCCGG GGTCGGGGAA CCCCGGGCCG GGGCTCGGGA GCCGGACTGG GTGTCTGGAG AACAGCGCCG AACTGGGGGA ACAGGCGGAT GTGGGGATCT GGGGTCAGGT GGCCTTCAAA CAGCTCGCTC CCGGCTGTGG GAAGTTCCAT CACTCTCCAA GCTTCATTCA CAATCCGGGC CTCAGTTTCT CCCACCGTCC AATTGGGGTT GGGGGGGGTC TTCAGTTCCA CAAGTCCGAA GTACTTCGAA TGGAGAGAGA AAGTGAGTGA GGGCGGGATG TTTAGTCTAG GAAGGGGCAC GCCCCCAGCC TGGTCCGTAC AGGGTCTAAC TAGCCCCAGT GATCGACGAG TTGGGGAGGC CGTCGAGAGG CACTAGGGTC TGTGCCCTCT ATACCATGTG GGGTGCCCCC TTCCATCCGG TGACTTCAGT GCCCAGCTAT GCGGGGGGAG GGACTGGGGT CGGGCCCGGT GCCCAGGAGT GAGTCACCCG CCGGCGGCAG GGGGGTTGTG GGGACCTGAC CCCCCCCACC CCCAGCGTCC CGCGCTACGC CCTCCCTCCC GGAGCTGCCG TGTCCCGGTC GGGCCGTGCG GTCACCTGTG

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_016639
• UniProt ID: Q9NP84
• Synonyms: CD266; FN14; TWEAKR
• Summary: Receptor for TNFSF12/TWEAK. Weak inducer of apoptosis in some cell types. Promotes angiogenesis and the proliferation of endothelial cells. May modulate cellular adhesion to matrix proteins.[UniProtKB/Swiss-Prot Function]