MGAT1 Human Gene Knockout Kit (CRISPR)

CAT#: KN200717LP

MGAT1 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 Luciferase-Puro donor, 1 scramble control
• Donor DNA: Luciferase-Puro
• Symbol: MGAT1
• Locus ID: 4245
• Components:

  KN200717G1, MGAT1 gRNA vector 1 in pCas-Guide CRISPR vector

  KN200717G2, MGAT1 gRNA vector 2 in pCas-Guide CRISPR vector

  KN200717LPD, donor DNA containing left and right homologous arms and Luciferase-Puro functional cassette.

  Homologous arm and Luciferase-Puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; Luciferase-Puro in green; Right arm in violet

  TGTTAAATAA CCCTGGAGAT GATGGGCAAC TTCGTTTTGC TCCTGACATT CGTGGGGTGC CTCTGGTGCT TCCCTGTTGG TAAGGGGTTA ACTGTAGCCC TGAGGTGGGA CATTTGATTT TAAAAATCAG TCATCTTGGG GCGCTTAGGT TAGAGGAATG GTAGGCAGAT GCTGTCACTC CTTGCCCCTC CCCTCCTCCT TCCCACCTGG AGGGGAAATG AAATCTGACA GGTAGAAAGA GGGGAGTTGG GGTTCTTTTT CTCTCTCCCT CCACCAGCAT CACTCTCTGC CTCTCCCTCA AAAATACGTT CCTGGGTCAG GATATATGTT GACTCCCTAG AGAGCTCTGG AGTCAACCTC CTGGCCTTCC TCCACCCTCA CTCTTGGCCT TTTCCTGCCC CCATTTCCTC TACCTGTGGG GCATGGAGCC ACGAGCCTTT GTGTGACGGT TTGCTTTCTC TCTCCTGTCT TTAGGTGCAT GGCTGCCTCC TAATCCCATA GTCCAGAGGA GGCATCCCTA GGACTGCGGG CAAGGGAGCC GGGCAAGCCC AGGGCAGCCT TGAACCGTCC CCTGGCCTGC CCTCCCCGGT GGGGGCCAGG TCGATGGCGA CCCCGCCAGC CTCACCCGGG AAGTGATTCG CCTGGCCCAA GACGCCGAGG TGGAGCTGGA GCGGCAGCGT GGGCTGCTGC AGCAGATCGG GGATGCCCTG TCGAGCCAGC GGGGGAGGGT GCCCACCGCG GCCCCTCCCG CCCAGCCGCG TGTGCCTGTG ACCCCCGCGC CGGCGGTGAT TCCCATCCTG GTCATCGCCT GTGACCGCAG CACTGTTCGG CGCTGCCTGG ACAAGCTGCT GCATTATCGG CCCTCGGCTG AGCTCTTCCC CATCATCGTT AGCCAGGACT GCGGGCACGA GGAGACGGCC CAGGCCATCG CCTCCTACGG CAGCGCGGTC ACGCACATCC GGCAGCCCGA CCTGAGCAGC ATTGCGGTGC CGCCGGACCA CCGCAAGTTC CAGGGCTACT ACAAGATCGC GCGCCACTAC CGCTGGGCGC TGGGCCAGGT CTTCCGGCAG TTTCGCTTCC CCGCGGCCGT GGTGGTGGAG GATGACCTGG AGGTGGCCCC GGACTTCTTC GAGTACTTTC GGGCCACCTA TCCGCTGCTG AAGGCCGACC CCTCCCTGTG GTGCGTCTCG GCCTGGAATG

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001114617, NM_001114618, NM_001114619, NM_001114620, NM_002406, NM_001364390, NR_157150, NR_157151, NM_001364379, NM_001364380, NM_001364384, NM_001364386, NM_001364388, NM_001364391, NM_001364392, NM_001364393, NM_001364394, NM_001364377, NM_001364381, NM_001364382, NM_001364383, NM_001364385, NM_001364387, NM_001364389, NM_001364395, NR_157152, NR_157153, NR_157154
• UniProt ID: P26572
• Synonyms: GLCNAC-TI; GLCT1; GLYT1; GNT-1; GNT-I; MGAT
• Summary: There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I is a medial-Golgi enzyme essential for the synthesis of hybrid and complex N-glycans. The protein, encoded by a single exon, shows typical features of a type II transmembrane protein. The protein is believed to be essential for normal embryogenesis. Several variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]