Syntaxin 3 (STX3) Human Gene Knockout Kit (CRISPR)

CAT#: KN200658

STX3 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: Syntaxin 3
• Locus ID: 6809
• Components:

  KN200658G1, Syntaxin 3 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: AAGGCCGTGAGTTTCGCCGC

  KN200658G2, Syntaxin 3 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: TGCCAGGAGGGGTGCTGCAT

  KN200658D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  ACTCGACTGC CACCCAATGT AGCCCACATC ACATTGTCTT ATTTGTTTTC GGGAGCTTGT CCCCGCCTAG CAAGGAGTCG GCTAAGAACT GGATCCTAGC GAGGAGCCCG GCACAGACAG CGAATGACCG CAGCCAGACA GTCGCTCTTG CTCTTCCTCG GCCCTGCGGC AGGATCCGCC GGTGCAGGGG CCTCTCCCCG GACTCCACGC GTGTCTGGAG GGCTCTCGGG TTAGGGAAGG GGGCTTTGGA GACGCCCCGG GCGGCCGGGC GGTGGCGGGA CGCGGGCCCT TTAAGAAGGA GCGAGGGGCG CGGCCAGGTA GGGGCGGGTC CAGGGCGGAT CAGCGCTGCG CCGGCGCCGG CCCGGGAGCC GGATTTGGAG CGCGAGGCGC CGGTGGGGGC GGAGGGGGCT GCGCGGCGGA GGCTCCCGTG GCCTCGGACG CTCCTCCTAG CTAGCGGCCG CCGCCCGCCG CCGCCTGCGC CTCCAGCTCC TTCGCCCCGG CGGGCCCGGC CGCCGCTTCC GGCAGCTCAC CTGGGAAGCG CTCACCTGGG ACGCGCTCAC CTGGGACGCG CTACCTGCCT CCGGGCGCCT GGGCTTCAGG AGCCGGAGGC TTGCCCTCCC CAAGCCCCCA CCGCTTCCCG CGCCGGTTCC GCACCCTCCC CTCCCCTCCC CTGCGGGGTC ATGTGCCCGG TGATTCATCG CTAGGCAATG ATTTTCCTGA ACGCCTCTAA CTCTCCCACC CCGCAAGCTG GACAGTGTCT GTCCAGAGCT GGTTCCCGAA GCGGGTGCTT GGGCCCGGGA GGTCAGGGGT CCCTTCACCA CCTGCCCACT GCTGCCTGGC ACTATGCCCA GGACCAGCCT CCTTCTCCCT TTCCTGTTTC TCTCCTGAGG TGCTACAGGC CTGGCTGCCC CTTCCCCCAG GGAGCAGCTC AGAGCTCTCT GGTGACCTCT GGAGCTGTGA GCCGGTCACA TGCTGGGATG GGTCAGCTCC TCTCCAACCG GTGGTCTGTA ATTGTTATCC CCGGGTGTCA TTGTCCCCCC CTCCCCCGCC GTATCTTTTG GAACAGCTGT GGTTAGGTCA AAGGATCTGA AGGTAGACTA CCTCCAGCCT TGGCTGCGCA AAAGCTGTAA AGCCTTAGGT TACTTACTGT GAGTCTCTGT TTCCTTATCT ATCAAATGGG AATGATCATA CCCTAGGAAT

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001178040, NM_004177
• UniProt ID: Q13277
• Synonyms: STX3A
• Summary: The gene is a member of the syntaxin family. The encoded protein is targeted to the apical membrane of epithelial cells where it forms clusters and is important in establishing and maintaining polarity necessary for protein trafficking involving vesicle fusion and exocytosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2010]