GRB2 Human Gene Knockout Kit (CRISPR)

CAT#: KN200469BN

GRB2 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 mBFP-Neo donor, 1 scramble control
• Donor DNA: mBFP-Neo
• Symbol: GRB2
• Locus ID: 2885
• Components:

  KN200469G1, GRB2 gRNA vector 1 in pCas-Guide CRISPR vector

  KN200469G2, GRB2 gRNA vector 2 in pCas-Guide CRISPR vector

  KN200469BND, donor DNA containing left and right homologous arms and mBFP-Neo functional cassette.

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_002086, NM_203506
• UniProt ID: P62993
• Synonyms: ASH; EGFRBP-GRB2; Grb3-3; MST084; MSTP084; NCKAP2
• Summary: The protein encoded by this gene binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the Sem5 gene of C.elegans, which is involved in the signal transduction pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]