NSUN5 Human Gene Knockout Kit (CRISPR)

CAT#: KN200144BN

NSUN5 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 mBFP-Neo donor, 1 scramble control
• Donor DNA: mBFP-Neo
• Symbol: NSUN5
• Locus ID: 55695
• Components:

  KN200144G1, NSUN5 gRNA vector 1 in pCas-Guide CRISPR vector

  KN200144G2, NSUN5 gRNA vector 2 in pCas-Guide CRISPR vector

  KN200144BND, donor DNA containing left and right homologous arms and mBFP-Neo functional cassette.

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001168347, NM_001168348, NM_018044, NM_148956
• UniProt ID: Q96P11
• Synonyms: NOL1; NOL1R; NSUN5A; p120; p120(NOL1); WBSCR20; WBSCR20A
• Summary: This gene encodes a member of an evolutionarily conserved family of proteins that may function as methyltransferases. This gene is located in a larger region of chromosome 7 that is deleted in Williams-Beuren syndrome, a multisystem developmental disorder. There are two pseudogenes for this gene located in the same region of chromosome 7. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]