PPME1 Human Gene Knockout Kit (CRISPR)

CAT#: KN200009BN

PPME1 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 mBFP-Neo donor, 1 scramble control
• Donor DNA: mBFP-Neo
• Symbol: PPME1
• Locus ID: 51400
• Components:

  KN200009G1, PPME1 gRNA vector 1 in pCas-Guide CRISPR vector

  KN200009G2, PPME1 gRNA vector 2 in pCas-Guide CRISPR vector

  KN200009BND, donor DNA containing left and right homologous arms and mBFP-Neo functional cassette.

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001271593, NM_016147
• UniProt ID: Q9Y570
• Synonyms: PME-1
• Summary: This gene encodes a protein phosphatase methylesterase localized to the nucleus. The encoded protein acts on the protein phosphatase-2A catalytic subunit and supports the ERK pathway through dephosphorylation of regulatory proteins. It plays a role in malignant glioma progression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2012]