Mouse Oas1a activation kit by CRISPRa

SKU GA215352

Oas1a CRISPRa kit - CRISPR gene activation of mouse 2'-5' oligoadenylate synthetase 1A

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Target Symbol: Oas1a
• Locus ID: 246730
• Components:

  GA215352G1, Oas1a gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA215352G2, Oas1a gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA215352G3, Oas1a gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.
• Shipping: Ambient

Reference Data

• RefSeq: NM_145211
• UniProt ID: P11928
• Synonyms: L3
• Summary: Interferon-induced, dsRNA-activated antiviral enzyme which plays a critical role in cellular innate antiviral response. In addition, it may also play a role in other cellular processes such as apoptosis, cell growth, differentiation and gene regulation. Synthesizes higher oligomers of 2'-5'-oligoadenylates (2-5A) from ATP which then bind to the inactive monomeric form of ribonuclease L (RNase L) leading to its dimerization and subsequent activation. Activation of RNase L leads to degradation of cellular as well as viral RNA, resulting in the inhibition of protein synthesis, thus terminating viral replication. Can mediate the antiviral effect via the classical RNase L-dependent pathway or an alternative antiviral pathway independent of RNase L.[UniProtKB/Swiss-Prot Function]