Mouse Ppp1r3b activation kit by CRISPRa

SKU GA215195

Ppp1r3b CRISPRa kit - CRISPR gene activation of mouse protein phosphatase 1, regulatory subunit 3B

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Target Symbol: Ppp1r3b
• Locus ID: 244416
• Components:

  GA215195G1, Ppp1r3b gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA215195G2, Ppp1r3b gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA215195G3, Ppp1r3b gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.
• Shipping: Ambient

Reference Data

• RefSeq: NM_177741, NM_001364450, NM_001364451, NM_001364452
• UniProt ID: Q8C767
• Synonyms: 6430576E21; AW821953; GL; PTG
• Summary: Acts as a glycogen-targeting subunit for phosphatase PP1. Facilitates interaction of the PP1 with enzymes of the glycogen metabolism and regulates its activity. Suppresses the rate at which PP1 dephosphorylates (inactivates) glycogen phosphorylase and enhances the rate at which it activates glycogen synthase and therefore limits glycogen breakdown. Its activity is inhibited by PYGL, resulting in inhibition of the glycogen synthase and glycogen phosphorylase phosphatase activities of PP1. Dramatically increases basal and insulin-stimulated glycogen synthesis upon overexpression in hepatocytes (By similarity).[UniProtKB/Swiss-Prot Function]